转铁蛋白

    
BR,98%

TRF

源叶
S12027 一键复制产品信息
11096-37-0
MFCD00131337
铁传递蛋白
货号 规格 价格 上海 北京 武汉 南京 购买数量
S12027-10mg BR,98% ¥120.00 2 - 1 3
S12027-50mg BR,98% ¥380.00 >10 1 - 2
S12027-100mg BR,98% ¥700.00 >10 2 6 1
S12027-500mg BR,98% ¥2800.00 3 1 - 3
产品介绍 参考文献(51篇) 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

介绍:转铁蛋白是血浆中主要的铁传递蛋白,为细胞分化和细胞代谢提供所需的铁。研究发现 所有的细胞生长都离不开转铁蛋白。不但维持正常的细胞代谢依赖于以生物活性形态存在的 铁,铁还是一些酶的辅助因子,比如 RNA 聚合酶,DNA 合成酶。同时也是血红蛋白的重要 组成部分。

转铁蛋白是血浆糖蛋白,从血浆中分离而来,牛转铁蛋白以两种分子形态存在,分子量 分别为 74KD 和 78KD。每个转铁蛋白分子通过 C 端和 N 端结构位点可以结合两分子铁离子, 所以转铁蛋白以三种形态存在:脱铁(不结合铁离子),饱和铁(结合两分子铁离子)和部 分饱和,在生理情况下,转铁蛋白分子仅有 1/3 被铁饱和。

使用说明: 转铁蛋白对于绝大部分细胞系在无血清培养基中生长是必需的,研究表明饱和铁形态的 转铁蛋白对于促进细胞生长是最有效的形态。对于杂交瘤,黏连细胞和悬浮细胞系,在细胞 培养过程中,应保持培养基中转铁蛋白浓度在 0.5-100μ g/ml。针对不同的细胞系,需要选 择最合适的浓度。

转铁蛋白冻干粉需要保存在冷藏状态下,使用之前再溶解。一般用 PBS 或者其它合适 的溶剂在室温下将转铁蛋白冻干粉溶解成储液,浓度在 0.5-2.0mg/ml,然后用无菌滤膜进行过 滤,滤液可在无菌条件下 2-8℃保存 30 天。

外观: 冻干粉末
储存条件: 2-8℃
注意: 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。

参考文献(51篇)

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