• 产品描述： MCU-i4 是 mitochondrial calcium uniporter (MCU) 复合物的负调节剂，可直接结合 MICU1 中的特定裂缝并减少线粒体 Ca2+ 流入

• 靶点： MCU1;MitochondrialMetabolism

• 体外研究：
  MCU-i4 decreases mitochondrial Ca2+ influx. Docking simulations reveal that MCU-i4 directly binds a specific cleft in MICU1, a key element of the MCU complex that controls channel gating. Accordingly, in MICU1-silenced or deleted cells, the inhibitory effect of MCU-i4 is lost. Moreover, MCU-i4 fails to inhibit mitochondrial Ca2+ uptake in cells expressing a MICU1 mutated in the critical amino acids that forge the predicted binding cleft.

• 细胞实验： Cell lines: C2C12 myoblasts Concentrations: 10 μM Incubation Time: 24 h Method: Growing medium of 90% confluent C2C12 myoblasts is replaced with DMEM containing 2%-heat inactivated horse serum (HS) (differentiation medium). Five days later, cells are incubated for 24 h with MCU-i4 (10 μM) or with DMSO alone (0.1% v/v). At the end of the experiment, myotubes images are taken and myotubes width is measured by means of ImageJ software.

• 参考文献：
  1. Giulia Di Marco, et al. A High-Throughput Screening Identifies MICU1 Targeting Compounds. Cell Rep. 2020 Feb 18;30(7):2321-2331.e6.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.651 ml	13.255 ml	26.51 ml
  5 mM	0.53 ml	2.651 ml	5.302 ml
  10 mM	0.265 ml	1.326 ml	2.651 ml
  50 mM	0.053 ml	0.265 ml	0.53 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *