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Taq酶 BR,2-5u/ul,99%

Taq DNA Polymerase

源叶
S10118
9012-90-2
MFCD00166026
品牌 货号 产品规格 价格(RMB) 库存(上海) 北京 武汉 南京 购买数量
源叶 S10118-1000U BR,2-5u/ul,99% ¥120.00元 6 2 2 1
源叶 S10118-10KU BR,2-5u/ul,99% ¥800.00元 1 - - -
产品介绍 参考文献(26篇) 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

     为重组 E. coli 菌株,其基因组中含有来源于 Thermusaquaticus YTI 中克隆的 Taq DNA polymerase 基因。本产品是一种热稳定性酶,分子量大约为 94kDa。它与天然 Tag DNA 聚合酶有相同的功能。在 Mg 2+存在的条件下可催化核苷酸沿 5’→3’方向发生聚合反应,形成双链 DNA;同时它还具有 5’ →3’外切酶活性。

 

    使用本品扩增得到的 PCR 产物的 3´端附有一个"A"碱基,因此可直接克隆于 T-Vector 中。

 

活性定义:在 74℃条件下,30 分钟内催化 10nmol 的 dNTP 掺入酸不溶性沉淀物所需要的酶量定义为一个活性单位。

 

贮存条件: 20 mM Tris-HC(l pH 8.0,25℃),100 mM KCl,0.1 mM EDTA,1 mM DTT,0.5% NP-40 ,0.5% Tween -20 ,50%Glycerol 

 

反应条件: 50mM KCl, 10 mM Tris-HCl (Ph8.6,25℃), 0.1% Triton X-100 ,1.5mM MgCl2. 

 

质量保证:本品经 PCR 验证,活性测定,内切核酸酶/缺刻酶测定和 SDS-PAGE 纯度鉴定。保证产品的质量稳定可靠。

 

使用注意:除了酶活性丧失外,下列因素也可能导致扩增失败

 

(1)模板太多或太少;(2)样品中存在 Mg 2+ 络合剂,导致 Mg 2+实际浓度过低;(3) PCR 仪温度不准; (4) 临床来源的样品中含有未知的 Taq 酶抑制剂; (5) 引物部分降解;(6) dNTP 部分降解;(7) Taq 酶用量太大等。

 

 建议:每 50 微升反应体系中,酶用量为 0.5-1 微升最好,酶量少可能扩增效率低下,使用时请注意! 

 

用途: 

 1) 常规 PCR 鉴定。

 2) 小片段目标基因克隆(推荐小于 500bp)。

 3) 标记 DNA 3’-末端。

 4) 平端 PCR 产物加“A”。

储存条件: -20℃
注意: 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。

参考文献(26篇)

21. [IF=4.06] Xianghui Zhang et al."MiR-199a-5p inhibition protects cognitive function of ischemic stroke rats by AKT signaling pathway."Am J Transl Res. 2020; 12(10): 6549–6558 20. [IF=4.238] Hong-Cheng Luo et al."Role of long noncoding RNA MEG3/miR-378/GRB2 axis in neuronal autophagy and neurological functional impairment in ischemic stroke."J Biol Chem. 2020 Oct;295(41):14125-14139 19. [IF=4.932] Jing Wang et al."MicroRNA-532-5p upregulation protects neurological deficits after ischemic stroke through inhibition of BTB and CNC homology 1."Int Immunopharmacol. 2021 Nov;100:108003 18. [IF=4.966] Na-Na Zhu et al."Autologous blood transfusion stimulates wound healing in diabetic mice through activation of the HIF‐1α pathway by improving the blood preservation solution."Faseb J. 2020 May;34(5):6038-6054 17. [IF=5.241] Ma Yu-Shui et al."Exosomal microRNA-15a from mesenchymal stem cells impedes hepatocellular carcinoma progression via downregulation of SALL4."Cell Death Discov. 2021 Aug;7(1):1-11 16. [IF=5.546] Ying Cai et al."Functional suppression of Epiregulin impairs angiogenesis and aggravates left ventricular remodeling by disrupting the extracellular‐signal‐regulated kinase1/2 signaling pathway in rats after acute myocardial infarction."J Cell Physiol. 20 15. [IF=5.59] Mao Lei et al."Elevation of miR-146a Inhibits BTG2/BAX Expression to Ameliorate Postoperative Cognitive Dysfunction Following Probiotics (VSL#3) Treatment."Mol Neurobiol. 2021 Jul;58(7):3457-3470 14. [IF=5.736] Minghuan Mao et al."OCT3/4 enhances tumor immune response by upregulating the TET1-dependent NRF2/MDM2 axis in bladder cancer."Genomics. 2021 Jul;113:2122 13. [IF=7.2] Yu-Shui Ma et al."M2 macrophage-derived exosomal microRNA-155-5p promotes the immune escape of colon cancer by downregulating ZC3H12B."Mol Ther-Oncolytics. 2021 Mar;20:484 12. [IF=2.939] Xue Yu-Long et al."Silencing of STAT4 Protects Against Autoimmune Myocarditis by Regulating Th1/Th2 Immune Response via Inactivation of the NF-κB Pathway in Rats."Inflammation. 2019 Aug;42(4):1179-1189 11. [IF=2.991] Luo Xiao-Yan et al."OSMR gene effect on the pathogenesis of chronic autoimmune Urticaria via the JAK/STAT3 pathway."Mol Med. 2018 Dec;24(1):1-13 10. [IF=3.098] Zhi‑Qiang Zhou et al."Adipose extracellular matrix promotes skin wound healing by inducing the differentiation of adipose‑derived stem cells into fibroblasts."Int J Mol Med. 2019 Feb;43(2):890-900 9. [IF=3.533] Liang-Liang Chen et al."SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/β-Catenin Pathway."Mol Cells. 2018 Sep 30; 41(9): 853–867 8. [IF=4.522] Zhen-Wei Ge et al."IRAK3 gene silencing prevents cardiac rupture and ventricular remodeling through negative regulation of the NF‐κB signaling pathway in a mouse model of acute myocardial infarction."J Cell Physiol. 2019 Jul;234(7):11722-11733 7. [IF=4.522] Ru‐Xue Bai et al."Repression of TXNIP–NLRP3 axis restores intestinal barrier function via inhibition of myeloperoxidase activity and oxidative stress in nonalcoholic steatohepatitis."J Cell Physiol. 2019 May;234(5):7524-7538 6. [IF=4.522] Hong-Cheng Fang et al."KRT1 gene silencing ameliorates myocardial ischemia–reperfusion injury via the activation of the Notch signaling pathway in mouse models."J Cell Physiol. 2019 Apr;234(4):3634-3646 5. [IF=6.508] Shi Chen et al."Silencing of long noncoding RNA LINC00958 prevents tumor initiation of pancreatic cancer by acting as a sponge of microRNA-330-5p to down-regulate PAX8."Cancer Lett. 2019 Apr;446:49 4. Yue, Rong-Chuan, et al. "Calpain silencing alleviates myocardial ischemia-reperfusion injury through the NLRP3/ASC/Caspase-1 axis in mice." Life sciences 233 (2019): 116631.https://doi.org/10.1016/j.lfs.2019.116631 3. Shao, Yuan, et al. "Contribution of microRNA-30d to the prevention of the thyroid cancer progression: mechanism and implications." (2020).10.21203/rs.3.rs-25018/v1 2. Liang, Tian-Song, et al. "MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2." Journal of experimental & clinical cancer research 38.1 (2019) 1. Tian, Y, Shen, L, Li, F, Yang, J, Wan, X, Ouyang, M. Silencing of RHEB inhibits cell proliferation and promotes apoptosis in colorectal cancer cells via inhibition of the mTOR signaling pathway. J Cell Physiol. 2019; 235: 442– 453. https://doi.org/10.1002/

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