十六烷基三甲基溴化铵

    
AR,99%

CTAB

源叶
S15001 一键复制产品信息
57-09-0
C19H42BrN
364.45
MFCD00011772
鲸蜡烷三甲基溴化铵;西曲溴铵;溴棕三甲铵
货号 规格 价格 上海 北京 武汉 南京 购买数量
S15001-100g AR,99% ¥60.00 >10 - - 2
S15001-500g AR,99% ¥198.00 >10 - 2 -
S15001-5*500g AR,99% ¥900.00 货期:2-3天 - - -
产品介绍 参考文献(46篇) 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

溴化十六烷基三甲胺:CTAB是一种阳离子去污剂,具有从低离子强度的溶液中沉淀核酸和酸性多聚糖的特性,在这种条件下,蛋白质和中性多聚糖仍留在溶液里,在高离子强度的溶液里,与蛋白质和大多数酸性多聚糖以外的多聚糖形成复合物,只是不能沉淀核酸。因此,CTAB可以用于从大量产生粘多糖的有机体如植物以及某些革兰氏阴性菌(包括E.coli的某些株)中制备纯化DNA 。
CTAB原理:CTAB是一种阳离子去污剂,具有从低离子强度的溶液中沉淀核酸和酸性多聚糖的特性,在这种条件下,蛋白质和中性多聚糖仍留在溶液里,在高离子强度的溶液里,能够与蛋白质和大多数酸性多聚糖以外的多聚糖形成复合物,只是不能沉淀核酸,从而将核酸分离出来。
CTAB使用方法:
1.提取DNA配方:
CTAB 4g
NaCl 16.364 g
1M Tris-HCl 20 mL( PH8.0)
冷却后0.2-1%2-巯基乙醇(400ul)氯仿-异戊醇(24:1):先加96ml氯仿,再加4ml异戊醇,摇匀即可。
2.提取RNA配方
2% CTAB(W/V)
2%聚乙烯吡咯烷酮PVP(W/V)
100 mM Tris-HCl(pH8.0,DEPC处理的水配制)
25mM EDTA
由于在高温灭菌条件下,Tris-HCl要和DEPC发生反应,所以配RNA提取缓冲液时直接用DEPC 处理的水
CTAB使用注意事项:具有刺激性,若吸入、摄入或皮肤吸收,会对人体有害。使用时要戴合适的手套和防护眼镜(或在通风橱操作)。避免吸入其粉尘。

熔点: 248-251℃
外观: 白色结晶
溶解性: Souble  in  EtOH
敏感性: 易吸潮
储存条件: RT
用途: 催化剂,乳化剂,消毒剂,杀菌基,抗静电剂等。比色测定或光度测定锑和锡。肠内葡萄糖吸收抑制剂。芳烃还原和烯环氧化时的相转移催化剂。
注意: 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。

参考文献(46篇)

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