• 介绍：
  溴化十六烷基三甲胺：CTAB是一种阳离子去污剂，具有从低离子强度的溶液中沉淀核酸和酸性多聚糖的特性，在这种条件下，蛋白质和中性多聚糖仍留在溶液里，在高离子强度的溶液里，与蛋白质和大多数酸性多聚糖以外的多聚糖形成复合物，只是不能沉淀核酸。因此，CTAB可以用于从大量产生粘多糖的有机体如植物以及某些革兰氏阴性菌(包括E.coli的某些株)中制备纯化DNA 。

  CTAB原理：CTAB是一种阳离子去污剂，具有从低离子强度的溶液中沉淀核酸和酸性多聚糖的特性，在这种条件下，蛋白质和中性多聚糖仍留在溶液里，在高离子强度的溶液里，能够与蛋白质和大多数酸性多聚糖以外的多聚糖形成复合物，只是不能沉淀核酸，从而将核酸分离出来。

  CTAB使用方法：

  1.提取DNA配方：

  CTAB 4g

  NaCl 16.364 g

  1M Tris-HCl 20 mL( PH8.0)

  冷却后0.2-1%2-巯基乙醇（400ul）氯仿-异戊醇（24：1）：先加96ml氯仿，再加4ml异戊醇，摇匀即可。

  2.提取RNA配方

  2% CTAB（W/V）

  2%聚乙烯吡咯烷酮PVP（W/V）

  100 mM Tris-HCl（pH8.0,DEPC处理的水配制）

  25mM EDTA

  由于在高温灭菌条件下，Tris-HCl要和DEPC发生反应，所以配RNA提取缓冲液时直接用DEPC 处理的水

  CTAB使用注意事项：具有刺激性，若吸入、摄入或皮肤吸收，会对人体有害。使用时要戴合适的手套和防护眼镜（或在通风橱操作）。避免吸入其粉尘。

• 熔点： 248-251℃

• 外观： 白色结晶

• 溶解性： Souble  in  EtOH

• 敏感性： 易吸潮

• 储存条件： RT

• 用途： 催化剂,乳化剂,消毒剂,杀菌基,抗静电剂等。比色测定或光度测定锑和锡。肠内葡萄糖吸收抑制剂。芳烃还原和烯环氧化时的相转移催化剂。

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *