产品描述: | SB 4 是一种 bone morphogenetic protein 4 (BMP4) signaling 的有效的选择性激动剂,其EC50值为74 nM。SB 4可增强经典的BMP信号传导并激活 SMAD-1/5/9 磷酸化 |
靶点: |
BMP4(Cell-free assay):74 nM(EC50);TGF-beta/Smad |
体外研究: |
BMP signaling agonist sb4 (0.05 μM-1 μM; 24 hours) induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions. BMP signaling agonist sb4 (1 μM; 0-60 mins) enhances the efficacy of signaling at each concentration of rhBMP4 tested. This effect is most pronounced at low concentrations of rhBMP4, whereby sb4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4. |
细胞实验: |
Cell lines: Primary mouse kidney epithelial cells (PRECs) Concentrations: 0.05 μM-1 μM Incubation Time: 0-1 h, 24 h Method: The BRE-Luc cells are stimulated with 2 ng/ml rhBMP4 for 1 h, after which the media are removed and replaced with fresh media containing either DMSO or 1 μm SB 4. p-SMAD-1/5/9 levels are then examined after 0, 5, 15, 30, 45, and 60 min of SB 4 treatment and compared with DMSO controls. |
参考文献: |
1. Bradford STJ, Ranghini EJ, Grimley E, Lee PH, Dressler GR. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds. J Biol Chem. 2019 Mar 1;294(9):3125-3136. |
溶解性: |
Soluble in DMSO、Ethanol |
保存条件: |
-20℃ |
配置溶液浓度参考: |
|
1mg |
5mg |
10mg |
1 mM |
3.123 ml |
15.615 ml |
31.23 ml |
5 mM |
0.625 ml |
3.123 ml |
6.246 ml |
10 mM |
0.312 ml |
1.562 ml |
3.123 ml |
50 mM |
0.062 ml |
0.312 ml |
0.625 ml |
|
注意: |
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