| 产品描述： | StemRegenin 1 is an aryl hydrocarbon receptor (AhR) inhibitor. |
| 靶点： |
AhR;Aryl Hydrocarbon Receptor;AhR;ArylHydrocarbonReceptor |
| 体外研究： |
在NOD.Cg-Prkdcscid Il2rgtm1Wj1 / SzJ（NSG）小鼠中,通过SR1促进CB CD34 +细胞的增殖,能够有助于在早期进行细胞植入及持续植入 |
| 体内研究： |
在体外,StemRegenin 1（1 μM）能够促进CD34 +细胞的增殖（EC 50=?120 nM）,同时降低VentX的表达水平。StemRegenin 1能够抑制光亲和配体（PAL）结合（IC 50 = 40 nM） |
| 细胞实验： |
StemRegenin 1 (SR1) is prepared in DMSO and stored, and then diluted with appropriate medium before use. A quantity of 250,000 CB-derived CD34+ cells are cultured with control conditions (DMSO, 0.01%) or StemRegenin 1 (0.75 μM) for 3 weeks. At this point control cultures had expanded 1080-fold and StemRegenin 1 treated cells expanded 2024-fold relative to starting cell numbers. A quantity of 30 to 30,000 uncultured CD34+ CB-derived cells or a fraction of the final culture equivalent to 30 to 10,000 starting cells are transplanted. The cells are injected intravenously via the retro-orbital route into sub-lethally irradiated (300 rads, 200 rads) 6- to 10-week-old NSG mice. Engraftment is performed within 24 h after irradiation. Engraftment is monitored by flow cytometric analysis of blood obtained via retro-orbital sinus or bone marrow using anti-human CD45 and anti-mouse CD45 antibodies. The mice are sacrificed between 13-16 weeks posttransplantation; bone marrow (from both femurs and tibiae), spleen and thymu |
| 参考文献： |
1. Gao H, et al. Suppression of homeobox transcription factor VentX promotes expansion of human hematopoietic stem/multipotent progenitor cells. J Biol Chem, 2012, 287(35), 219979-291987. 2. Gori JL, et al. Efficient generation, purification, and expansion of CD34(+) hematopoietic progenitor cells from nonhuman primate-induced pluripotent stem cells. Blood, 2012, 120(13), e35-44. 3. Jin H, Ji C, Ren F, et al. AHR-Mediated Oxidative Stress Contributes to the Cardiac Developmental Toxicity of Trichloroethylene in Zebrafish Embryos[J]. Journal of Hazardous Materials. 2020, 385: 121521. 4. Boitano AE, et al. Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells. Science, 2010, 329(5997), 1345-1348. |
| 溶解性： |
soluble in DMSO |
| 保存条件： |
-20℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.328 ml |
11.64 ml |
23.281 ml |
| 5 mM |
0.466 ml |
2.328 ml |
4.656 ml |
| 10 mM |
0.233 ml |
1.164 ml |
2.328 ml |
| 50 mM |
0.047 ml |
0.233 ml |
0.466 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京