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• 产品描述： StemRegenin 1 is an aryl hydrocarbon receptor (AhR) inhibitor.

• 靶点： AhR;Aryl Hydrocarbon Receptor;AhR;ArylHydrocarbonReceptor

• 体外研究：
  在NOD.Cg-Prkdcscid Il2rgtm1Wj1 / SzJ（NSG）小鼠中,通过SR1促进CB CD34 +细胞的增殖,能够有助于在早期进行细胞植入及持续植入

• 体内研究：
  在体外,StemRegenin 1（1 μM）能够促进CD34 +细胞的增殖（EC 50=?120 nM）,同时降低VentX的表达水平。StemRegenin 1能够抑制光亲和配体（PAL）结合（IC 50 = 40 nM）

• 细胞实验： StemRegenin 1 (SR1) is prepared in DMSO and stored, and then diluted with appropriate medium before use. A quantity of 250,000 CB-derived CD34+ cells are cultured with control conditions (DMSO, 0.01%) or StemRegenin 1 (0.75 μM) for 3 weeks. At this point control cultures had expanded 1080-fold and StemRegenin 1 treated cells expanded 2024-fold relative to starting cell numbers. A quantity of 30 to 30,000 uncultured CD34+ CB-derived cells or a fraction of the final culture equivalent to 30 to 10,000 starting cells are transplanted. The cells are injected intravenously via the retro-orbital route into sub-lethally irradiated (300 rads, 200 rads) 6- to 10-week-old NSG mice. Engraftment is performed within 24 h after irradiation. Engraftment is monitored by flow cytometric analysis of blood obtained via retro-orbital sinus or bone marrow using anti-human CD45 and anti-mouse CD45 antibodies. The mice are sacrificed between 13-16 weeks posttransplantation; bone marrow (from both femurs and tibiae), spleen and thymu

• 参考文献：
  1. Gao H, et al. Suppression of homeobox transcription factor VentX promotes expansion of human hematopoietic stem/multipotent progenitor cells. J Biol Chem, 2012, 287(35), 219979-291987. 2. Gori JL, et al. Efficient generation, purification, and expansion of CD34(+) hematopoietic progenitor cells from nonhuman primate-induced pluripotent stem cells. Blood, 2012, 120(13), e35-44. 3. Jin H, Ji C, Ren F, et al. AHR-Mediated Oxidative Stress Contributes to the Cardiac Developmental Toxicity of Trichloroethylene in Zebrafish Embryos[J]. Journal of Hazardous Materials. 2020, 385: 121521. 4. Boitano AE, et al. Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells. Science, 2010, 329(5997), 1345-1348.

• 溶解性： soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.328 ml	11.64 ml	23.281 ml
  5 mM	0.466 ml	2.328 ml	4.656 ml
  10 mM	0.233 ml	1.164 ml	2.328 ml
  50 mM	0.047 ml	0.233 ml	0.466 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *