| 产品描述： | ABT-751 has been investigated for the treatment of Lung Cancer, Non-Small Cell Lung Cancer, and Non-Small-Cell Lung Cancer. |
| 靶点： |
Autophagy Microtubule Associated;MicrotubuleAssociated;Autophagy |
| 体内研究： |
ABT-751显示出对动态微管的选择性作用并且保留稳定的微管,从而解释了在ABT-751的IC90浓度下乙酰化和去酪氨酸α-微管蛋白阳性聚合小管的持久性。在体外,ABT-751显示选择性细胞毒性,在神经母细胞瘤中IC50为0.6-2.6 μM,在其他实体瘤细胞系中为0.7-4.6 μM |
| 细胞实验： |
Cells, in 1640 RPMI media with FBS, are plated in triplicate onto 96 well tissue culture plates in numbers determined optimal for confluent monolayer growth (5,000 cells/well for HOS, HTB-186 Daoy; 10,000 cells/well for TC-71, RD, SK-N-AS, SK-N-DZ, LD; 30,000 cells/well for KCNR), with an automated, multichannel pipette system. Cells are incubated for 24 hours at 37 °C/5% CO2 then exposed to vehicle control (1.25% DMSO/Water), VCR (0.1–1000 nM), ABT-751 (0.1 nM–100 μM), and in 4 cell lines (SK-N-AS, KCNR, RD, TC-71) combretastatin (0.1–1000 nM) for 72 hours. Cells are fixed with trichloroacetic acid (final concentration 10%) at 4 °C, washed, then dried at room temperature, stained with SRB in 1% acetic acid and dye is then solubilized with Tris base. Optical density measurements are performed at 540 and 405 nm dual wavelengths in a Bio-Tek EL 340 UV plate reader. (Only for Reference) |
| 参考文献： |
1.Meany HJ, et al. Pediatr Blood Cancer. 2010, 54(1), 47-54. 2.Jorgensen TJ, et al. Cancer Chemother Pharmacol. 2007, 59(6), 725-732. 3.Silver M, et al. J Vet Intern Med. 2012, 26(2), 349-354. |
| 溶解性： |
DMSO:37.1 mg/mL (100 mM) Ethanol:9.3 mg/mL (25 mM) |
| 保存条件： |
-20℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.692 ml |
13.462 ml |
26.924 ml |
| 5 mM |
0.538 ml |
2.692 ml |
5.385 ml |
| 10 mM |
0.269 ml |
1.346 ml |
2.692 ml |
| 50 mM |
0.054 ml |
0.269 ml |
0.538 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京