| 产品描述： | WS3, a β cell proliferation inducer, regulates Erb3 binding protein-1 (EBP1) and the IκB kinase pathway |
| 靶点： |
EGFR;IκB/IKK |
| 体外研究： |
WS3 induces pancreatic R7T1 β cell proliferation in dose response, with an EC50 value of 0.28 μM.
WS3 (1.0 nM-1.0 µM) reversibly proliferate primary retinal pigment epithelial (RPE) cells isolated from fetal and adult human donors. Following withdrawal of WS3, RPE cells differentiate into a functional monolayer, as exhibited by their expression of mature RPE genes and phagocytosis of photoreceptor outer segments. |
| 体内研究： |
在小鼠β细胞系R7T1中,WS3诱导细胞增殖（EC50=0.074 μM）。在原代胚胎和成人视网膜色素上皮细胞中,WS3可逆诱导细胞发生增殖 |
| 细胞实验： |
R7T1 cells are growth-arrested by removal of doxycycline for 2 days and plated into 384-well plates at a density of 3,000 cells/well in growth medium. Β cell proliferation is assessed using CellTiter Glo after 4 days. Fold increase in cell number is calculated by normalizing compound-treated wells to the median of DMSO-treated wells. (Only for Reference) |
| 参考文献： |
1. Shen W, et al. Small-molecule inducer of β cell proliferation identified by high-throughput screening. J Am Chem Soc. 2013 Feb 6;135(5):1669-72. 2. Swoboda JG, et al. Small molecule mediated proliferation of primary retinal pigment epithelial cells. ACS Chem Biol. 2013, 8(7), 1407-1411. |
| 溶解性： |
Soluble in DMSO、Ethanol |
| 保存条件： |
-20℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
1.756 ml |
8.778 ml |
17.557 ml |
| 5 mM |
0.351 ml |
1.756 ml |
3.511 ml |
| 10 mM |
0.176 ml |
0.878 ml |
1.756 ml |
| 50 mM |
0.035 ml |
0.176 ml |
0.351 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京