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• 产品描述： CCT007093 is an effective protein phosphatase 1D (PPM1D Wip1) inhibitor. Wip1 inhibition can activate the mTORC1 pathway and enhance hepatocyte proliferation after hepatectomy

• 靶点： Phosphatase;Apoptosis;Autophagy;Apoptosis;Phosphatase;Autophagy

• 体外研究：
  CCT007093 (25 or 50 µM, 8 h) significantly increases the phosphorylation levels of mTOR at Ser2448, Ser2481 and Ser2159, and the phosphorylation levels of p70S6K (Thr389) and S6 (Ser235/236) are also up-regulated in transfected HEK293T cell line. CCT007093 shows specificity for MCF-7 cells, reducing viability by 40% after 2 days with no observable effect on the growth of HeLa cells. CCT007093 induces P38 phosphorylation at 4 h post exposure in MCF-7 cells (sensitive to PPM1D inhibition), and does not induce P38 phosphorylation in HeLa cells (relatively resistant to PPM1D inhibition). Western Blot Analysis Cell Line: HEK293T cell line. Concentration: 25 or 50 µM. Incubation Time: 8 h. Result: The phosphorylation levels of mTOR at Ser2448, Ser2481 and Ser2159 were all significantly increased. The phosphorylation levels of p70S6K (Thr389) and S6 (Ser235/236) were also up-regulated

• 体内研究：
  CCT007093 (6.4 mg/kg) enhances liver regeneration and increases the survival rate of mice following major hepatectomy. Animal Model: Wild-type mice. Dosage: 3.2 and 6.4 mg/kg. Administration: Injected intraperitoneally 4 times (mice were sacrificed at 36 h post-PH). Result: Significantly improved survival in mice following major hepatectomy (80% hepatectomy).The level of PCNA was also significantly increased in the liver of CCT007093-treated mice at 36h, 48h and 72h post-PH.

• 参考文献：
  1. Lingling Zhang, et al. Inhibition of wild-type p53-induced phosphatase 1 promotes liver regeneration in mice by direct activation of mammalian target of rapamycin. Hepatology. 2015 Jun;61(6):2030-41. 2. S Rayter, et al. A chemical inhibitor of PPM1D that selectively kills cells overexpressing PPM1D. Oncogene. 2008 Feb 14;27(8):1036-44.

• 溶解性： Soluble  in  DMSO、DMF

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	3.671 ml	18.356 ml	36.713 ml
  5 mM	0.734 ml	3.671 ml	7.343 ml
  10 mM	0.367 ml	1.836 ml	3.671 ml
  50 mM	0.073 ml	0.367 ml	0.734 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *