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• 产品描述： Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK)是一种特异性Caspase-8抑制剂。Z-IETD-FMK也是颗粒酶B（granzyme B）抑制剂

• 靶点： Caspase-8;Caspase

• 体外研究：
  Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures.

• 体内研究：
  In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries.

• 细胞实验： Cell lines: Rat neonatal cardiomyocytes Concentrations: 50 µM Incubation Time: 24 h Method: Drugs were added to the cell culture medium simultaneously with ceramide, and surviving cells were counted 24 hours later.

• 动物实验： Animal Models: T. cruzi-infected mice Dosages: 0.4 mg/3 days Administration: --

• 参考文献：
  1. Tezel G, et al. Inhibition of caspase activity in retinal cell apoptosis induced by various stimuli in vitro. Invest Ophthalmol Vis Sci. 1999, 40(11), 2660-2667. 2. Wang J, et al. Involvement of caspase 3- and 8-like proteases in ceramide-induced apoptosis of cardiomyocytes. J Card Fail. 2000, 6(3), 243-249. 3. Silva EM, et al. Caspase-8 activity prevents type 2 cytokine responses and is required for protective T cell-mediated immunity against Trypanosoma cruzi infection. J Immunol. 2005, 174(10), 6314-6321. 4. Monnier PP, et al. Involvement of caspase-6 and caspase-8 in neuronal apoptosis and the regenerative failure of injured retinal ganglion cells. J Neurosci. 2011, 31(29), 10494-10505.

• 溶解性： soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	1.527 ml	7.637 ml	15.275 ml
  5 mM	0.305 ml	1.527 ml	3.055 ml
  10 mM	0.153 ml	0.764 ml	1.527 ml
  50 mM	0.031 ml	0.153 ml	0.305 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *