| 产品描述： | FCCP, a potent uncoupler of oxidative phosphorylation in mitochondria, transport protons across cell membranes leading to disrupts ATP synthesis. |
| 靶点： |
ATPase;Mitochondrial Metabolism;ATPase;MitochondrialMetabolism |
| 体内研究： |
FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although FCCP-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype |
| 细胞实验： |
Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times. (Only for Reference) |
| 参考文献： |
1. Guimarães EL, et al. BMC Gastroenterol. 2012, 12:68. 2.Zander-Fox DL, et al. Biol Reprod. 2015, 92(5):124. 3.Shi J, Zhang F, Chen L, et al. Systemic mitochondrial disruption is a key event in the toxicity of bacterial pore‐forming toxins to Caenorhabditis elegans[J]. Environmental Microbiology. 4.Muñoz F, et al. FEBS Lett. 2001, 492(1-2):156-159. |
| 溶解性： |
DMSO:25.4 mg/mL (100 mM) |
| 保存条件： |
2-8℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
3.934 ml |
19.672 ml |
39.344 ml |
| 5 mM |
0.787 ml |
3.934 ml |
7.869 ml |
| 10 mM |
0.393 ml |
1.967 ml |
3.934 ml |
| 50 mM |
0.079 ml |
0.393 ml |
0.787 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京