| 产品描述： | BML-284 is a potent and cell-permeable Wnt signaling activator. BML-284 induces TCF-dependent transcriptional activity with an EC50 of 700 nM |
| 靶点： |
EC50: 700 nM (TCF-dependent transcriptional activity);Wnt/beta-catenin |
| 体外研究： |
BML-284 (10 µM; 24 hours) significantly increases the migration and invasion of both MNK45 and AGS cells and partially restores the migratory and invasive abilities of cells inhibited by pizotifen. BML-284 (10 µM; 24 hours) induces the expression of β-catenin significantly when compared with the NC group. It also partially reverses the effects induced by pizotifen on E-cadherin and N-cadherin expression in MNK45 and AGS cells compared with the pizotifen-treated group. Western Blot Analysis Cell Line: Human gastric cancer cell lines MNK45 and AGS Concentration: 10 µM Incubation Time: 24 hours Result: Induced β-catenin expression and reserved E-cadherin and N-cadherin expression in MNK45 and AGS cells. |
| 体内研究： |
BML-284 (10 ng) combines with Pyrimethanil (4 mg/L) could partially rescue the malformed phenotype and cardiac defects induced by Pyrimethanil in Tg (myl7:EGFP) transgenic embryos at 5.5 hpf are transferred into plates with 20 embryos |
| 参考文献： |
1. Liu J, et al. A small-molecule agonist of the Wnt signaling pathway. Angew Chem Int Ed Engl. 2005 Mar 18;44(13):1987-90. 2. Ying Jiang, et al. Pizotifen inhibits the proliferation and invasion of gastric cancer cells. Exp Ther Med. 2020 Feb;19(2):817-824. 3. Yunlong Meng, et al. Exposure to pyrimethanil induces developmental toxicity and cardiotoxicity in zebrafish. Chemosphere. 2020 Sep;255:126889. |
| 溶解性： |
soluble in DMSO |
| 保存条件： |
-20℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.854 ml |
14.271 ml |
28.541 ml |
| 5 mM |
0.571 ml |
2.854 ml |
5.708 ml |
| 10 mM |
0.285 ml |
1.427 ml |
2.854 ml |
| 50 mM |
0.057 ml |
0.285 ml |
0.571 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京