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• 产品描述： Mibefradil dihydrochloride is an blocker of calcium channel with moderate selectivity for T-type Ca2+ channels (T-type and L-type currents with IC50s of 2.7 μM and 18.6 μM, respectively).

• 靶点： Calcium Channel;CalciumChannel

• 体内研究：
  Compared with the saline-treated group, rats receiving Mibefradil or Ethosuximide show significant lower CaV3.2 expression in the spinal cord and DRG.

• 细胞实验： Human Orai1-3 cDNAs in tetracycline-regulated pcDNA4/TO vectors were transfected into HEK293 T-REx cells with stromal interaction molecule 1 (STIM1) stable expression. The Orai currents were recorded by whole-cell and excised-membrane patch clamp. Ca2+influx or release was measured by Fura-PE3/AM. Cell growth and death were monitored by WST-1, LDH assays and flow cytometry

• 参考文献：
  1. Shiue SJ, et al. Chronic intrathecal infusion of T-type calcium channel blockers attenuates CaV3.2 upregulation in nerve-ligated rats. Acta Anaesthesiol Taiwan. 2016 Oct 17. pii: S1875-4597(16)30071-6. 2. Li P , Rubaiy H N , Gui‐Lan Chen, et al. Mibefradil, a T‐type Ca2+ channel blocker also blocks Orai channels by action at the extracellular surface[J]. British Journal of Pharmacology, 2019, 176. 3. Brain KL, et al. The sources and sequestration of Ca(2+) contributing to neuroeffector Ca(2+) transients in the mouse vas deferens. J Physiol. 2003 Dec 1;553(Pt 2):627-35. 4. Yu YF, et al. Protection of the cochlear hair cells in adult C57BL/6J mice by T-type calcium channel blockers. Exp Ther Med. 2016 Mar;11(3):1039-1044.

• 溶解性： Soluble  in  H2O

• 保存条件： -20℃;充氩;干燥

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	1.759 ml	8.794 ml	17.589 ml
  5 mM	0.352 ml	1.759 ml	3.518 ml
  10 mM	0.176 ml	0.879 ml	1.759 ml
  50 mM	0.035 ml	0.176 ml	0.352 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *