| 产品描述： | JNK-IN-7 is a selective JNK1/2/3 inhibitor (IC50: 1.54/1.99/0.75 nM). It can also inhibit phosphorylation of c-Jun, which is a substrate of JNK kinase. |
| 靶点： |
JNK |
| 细胞实验： |
JNK-IN-7 is prepared in DMSO and stored, and then diluted with appropriate medium before use. Intestinal epithelial cell line (HCT116) is cultured in DMEM medium, supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100U/mL) and streptomycin (100g/mL), 2mM L-gentamycin, and 50μM 2-ME. These cells are stimulated with TNF (20ng/mL), LPS (100ng/mL), and IFN-γ (20ng/mL), respectively. After 24 or 48h of culture, cells are harvested followed by extraction of total RNA, and the levels of DMT1 mRNA are analyzed by qRT-PCR. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1μM), NF-κB inhibitor (BAY 11-7082, 1μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50μM) are also added into the culture medium. After 48h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR |
| 参考文献： |
1. Wu W, et al. Divalent metal-ion transporter 1 is decreased in intestinal epithelial cells and contributes to the anemia in inflammatory bowel disease. Sci Rep. 2015 Nov 17;5:16344. 2. Xu P, Zeng S, Xia X, et al. FAM172A promotes follicular thyroid carcinogenesis and may be a marker of FTC[J]. Endocrine-Related Cancer. 2020, 1(aop). 3. Zhang T, et al. Discovery of Potent and Selective Covalent Inhibitors of JNK. Chem Biol. 2012 Jan 27;19(1):140-54. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-20℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.026 ml |
10.13 ml |
20.261 ml |
| 5 mM |
0.405 ml |
2.026 ml |
4.052 ml |
| 10 mM |
0.203 ml |
1.013 ml |
2.026 ml |
| 50 mM |
0.041 ml |
0.203 ml |
0.405 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京