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• 产品描述： JNK-IN-7 is a selective JNK1/2/3 inhibitor (IC50: 1.54/1.99/0.75 nM). It can also inhibit phosphorylation of c-Jun, which is a substrate of JNK kinase.

• 靶点： JNK

• 细胞实验： JNK-IN-7 is prepared in DMSO and stored, and then diluted with appropriate medium before use. Intestinal epithelial cell line (HCT116) is cultured in DMEM medium, supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100U/mL) and streptomycin (100g/mL), 2mM L-gentamycin, and 50μM 2-ME. These cells are stimulated with TNF (20ng/mL), LPS (100ng/mL), and IFN-γ (20ng/mL), respectively. After 24 or 48h of culture, cells are harvested followed by extraction of total RNA, and the levels of DMT1 mRNA are analyzed by qRT-PCR. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1μM), NF-κB inhibitor (BAY 11-7082, 1μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50μM) are also added into the culture medium. After 48h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR

• 参考文献：
  1. Wu W, et al. Divalent metal-ion transporter 1 is decreased in intestinal epithelial cells and contributes to the anemia in inflammatory bowel disease. Sci Rep. 2015 Nov 17;5:16344. 2. Xu P, Zeng S, Xia X, et al. FAM172A promotes follicular thyroid carcinogenesis and may be a marker of FTC[J]. Endocrine-Related Cancer. 2020, 1(aop). 3. Zhang T, et al. Discovery of Potent and Selective Covalent Inhibitors of JNK. Chem Biol. 2012 Jan 27;19(1):140-54.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.026 ml	10.13 ml	20.261 ml
  5 mM	0.405 ml	2.026 ml	4.052 ml
  10 mM	0.203 ml	1.013 ml	2.026 ml
  50 mM	0.041 ml	0.203 ml	0.405 ml

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输入产品批号：

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *