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• 产品描述： MCC950 (CP-456773, CRID3) 是一种有效的 NLRP3 (NOD-like receptor (NLR) family, pyrin domain-containing protein 3) 的选择性抑制剂，在BMDMs和HMDMs中对应的IC50值分别为7.5 nM和8.1 nM

• 靶点： NLRP3(BMDM-based assay):7.5 nM; NLRP3(HMDM-based assay):8.1 nM;NOD

• 体外研究：
  MCC950 specifically inhibits NLPR3 inflammasome activation

• 体内研究：
  Inhibition of NLRP3 with MCC950 ameliorates proteinuria, renal histological lesions and podocyte foot process effacement in lupus-prone mice

• 细胞实验： Cell lines: NZM2328 podocyte cell line Concentrations: 1 µM Incubation Time: 24 h Method: The NZM2328 podocyte cell line was stimulated with a control serum or a serum from diseased NZM2328 mice or serum from diseased NZM2328 mice depleted of IgG, with or without MCC950 or Mito TEMPO for 24 hours. After this incubation, cells were subjected to flow cytometry and Western blot analyses for NLRP3 inflammasome activation, mitochondrial membrane potential and production of reactive oxygen species (ROS) analysis.

• 动物实验： Animal Models: female NZM2328, a new NZM strain produced by extended backcrosses of NZB/WF1 to NZW mice Dosages: 10mg/kg Administration: i.p.

• 参考文献：
  1. Fu R, et al. Podocyte Activation of NLRP3 Inflammasomes Contributes to the Development of Proteinuria in Lupus Nephritis. Arthritis Rheumatol. 2017 Aug;69(8):1636-1646. 2. Joshi H, et al. L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis. Cell Rep. 2022 Mar 15;38(11):110507. 3. Xie H, et al. Effects of mitochondrial reactive oxygen species-induced NLRP3 inflammasome activation on trichloroethylene-mediated kidney immune injury. Ecotoxicol Environ Saf. 2022 Oct 1;244:114067.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.472 ml	12.362 ml	24.723 ml
  5 mM	0.494 ml	2.472 ml	4.945 ml
  10 mM	0.247 ml	1.236 ml	2.472 ml
  50 mM	0.049 ml	0.247 ml	0.494 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *