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产品描述： IRAK-1-4 Inhibitor I is a dual inhibitor of IRAK4 and IRAK1. |
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靶点：
IRAK |
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细胞实验：
IRAK-1-4 Inhibitor I is dissolved in DMSO and stored, and then diluted with appropriate media before use. NCM460 cells, grown in 24-well plates, are incubated with 50 μM IRAK-1/4 inhibitor for 2 h. After 2 h, the media are changed, and new media with or without LPS (10 ng/mL) added. Treatment is terminated at 6 h, and spent media and cells are collected for IL-8 and other assays |
参考文献：
1.Powers JP, et al. Discovery and initial SAR of inhibitors of interleukin-1 receptor-associated kinase-4. Bioorg Med Chem Lett. 2006 Jun 1;16(11):2842-2845. 2.Bhattacharyya S, et al. Bcl10 mediates LPS-induced activation of NF-kappaB and IL-8 in human intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2007 Aug;293(2):G429-37. 3.Wu X, Xu M, Liu Y, et al. Dual Pharmacological Inhibition of IRAK1 and IRAK4 Prevents LPS Induced Monocyte Adhesion to Endothelial Cells[J]. bioRxiv. 2021 |
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溶解性：
Soluble in DMSO |
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保存条件：
-20℃ |
配置溶液浓度参考：
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1mg |
5mg |
10mg |
| 1 mM |
2.529 ml |
12.645 ml |
25.29 ml |
| 5 mM |
0.506 ml |
2.529 ml |
5.058 ml |
| 10 mM |
0.253 ml |
1.264 ml |
2.529 ml |
| 50 mM |
0.051 ml |
0.253 ml |
0.506 ml |
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| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
北京