| 产品描述： | SRI-011381是一种新型的TGF-beta信号通路激动剂，用来治疗阿尔茨海默病 |
| 靶点： |
TGF-β;TGF-beta/Smad |
| 体内研究： |
SRI-011381, a small-molecule agonist of TGFβ signaling, has no effect on outflow tract (OFT) expansion in zebrafish, suggesting that hyperactivation of TGFβ signaling is not sufficient to induce aneurysm |
| 细胞实验： |
Cell lines: pulmonary fibroblasts of silicosis mouse model Concentrations: 10 µM Incubation Time: -- Method: The tissues are extracted in a sterile environment, rinsed in PBS 3 times, and then cut into 1-mm3 tissue blocks. After repeated digestion with 0.25% trypsin at 37 ℃, the histolysate is evenly spread in a 10-cm petri dish. A high-glucose medium containing 10% fetal bovine serum, penicillin, and streptomycin is added to the petri dish. The cells are cultured in an incubator, and the medium is exchanged every 2-3 days. When the cells reached 80-90% confluence, they are passaged according to the ratio of 1: 3, and the fifth passage cells are taken for experimental study. Pulmonary fibroblasts are identified using anti-vimentin antibody with fluorescent secondary antibodies under a fluorescence microscope. For in vitro experiments, a range of concentrations of SF solution (0.04, 0.1, 0.18 and 0.28 mg/mL) are prepared for the treatment of fibroblasts. A 10 μM SRI-011381 solution is used for the activati |
| 动物实验： |
Animal Models: Zebrafish carrying ltbp3fb28 and ltbp1fb29 Dosages: 500 µM Administration: exposed |
| 参考文献： |
1. Hu L,et al.Exp Eye Res. 2019;182:44-56. 2. Han, Jingyin et al. Medical science monitor : international medical journal of experimental and clinical research vol. 27 e927978. 15 Jun. 2021, 3. Abrial M, et al. Dis Model Mech. 2022 Mar 1;15(3):dmm046979. |
| 溶解性： |
Soluble in DMSO、Ethanol |
| 保存条件： |
-20℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
3.035 ml |
15.175 ml |
30.351 ml |
| 5 mM |
0.607 ml |
3.035 ml |
6.07 ml |
| 10 mM |
0.304 ml |
1.518 ml |
3.035 ml |
| 50 mM |
0.061 ml |
0.304 ml |
0.607 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京