• 产品描述： ZM223 is a potent non-covalent NEDD8 activating enzyme (NAE) inhibitor, orally active

• 靶点： NEDD8 activating enzyme (NAE);SerineProtease

• 体外研究：
  ZM223 (0.1-1 μM; 4 hours) inhibits both HCT-116 and U-2OS cancer cells with IC50s of 100 and 122 nM, respectively. ZM223 (0.1-1 μM; 4 hours) causes a dose-response decrease in the level of NEDD8 and accumulation of the UBC12 protein, indicating the decrease of the subsequent NEDD8-UBC12 complex. Cell Viability Assay Cell Line: HCT116 colon cancer cells and U-2OS osteosarcoma cells Concentration: 0.1 μM, 1 μM Incubation Time: 4 hours Result: Inhibited both HCT-116 and U-2OS cancer cells. Western Blot Analysis Cell Line: HCT116 colon cancer cells Concentration: 0.1 μM, 1 μM Incubation Time: 4 hours Result: Caused a decrease in the level of NEDD8 and an increase in the downstream UBC12 protein.

• 参考文献：
  1. Ma H, Zhuang C, Xu X, et al. Discovery of benzothiazole derivatives as novel non-sulfamide NEDD8 activating enzyme inhibitors by target-based virtual screening. Eur J Med Chem. 2017;133:174-183. doi:10.1016/j.ejmech.2017.03.076

• 溶解性： Soluble  in  DMSO

• 保存条件： -20°C

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	1.99 ml	9.95 ml	19.899 ml
  5 mM	0.398 ml	1.99 ml	3.98 ml
  10 mM	0.199 ml	0.995 ml	1.99 ml
  50 mM	0.04 ml	0.199 ml	0.398 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *