| 产品描述： | ZM223 is a potent non-covalent NEDD8 activating enzyme (NAE) inhibitor, orally active |
| 靶点： |
NEDD8 activating enzyme (NAE);SerineProtease |
| 体外研究： |
ZM223 (0.1-1 μM; 4 hours) inhibits both HCT-116 and U-2OS cancer cells with IC50s of 100 and 122 nM, respectively. ZM223 (0.1-1 μM; 4 hours) causes a dose-response decrease in the level of NEDD8 and accumulation of the UBC12 protein, indicating the decrease of the subsequent NEDD8-UBC12 complex. Cell Viability Assay Cell Line: HCT116 colon cancer cells and U-2OS osteosarcoma cells Concentration: 0.1 μM, 1 μM Incubation Time: 4 hours Result: Inhibited both HCT-116 and U-2OS cancer cells. Western Blot Analysis Cell Line: HCT116 colon cancer cells Concentration: 0.1 μM, 1 μM Incubation Time: 4 hours Result: Caused a decrease in the level of NEDD8 and an increase in the downstream UBC12 protein. |
| 参考文献： |
1. Ma H, Zhuang C, Xu X, et al. Discovery of benzothiazole derivatives as novel non-sulfamide NEDD8 activating enzyme inhibitors by target-based virtual screening. Eur J Med Chem. 2017;133:174-183. doi:10.1016/j.ejmech.2017.03.076 |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-20°C |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
1.99 ml |
9.95 ml |
19.899 ml |
| 5 mM |
0.398 ml |
1.99 ml |
3.98 ml |
| 10 mM |
0.199 ml |
0.995 ml |
1.99 ml |
| 50 mM |
0.04 ml |
0.199 ml |
0.398 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京