• 介绍：

  罗丹明123 (Rhodamine 123、罗丹明123, Rh 123)是一种常用的阳离子绿色荧光染料，可以透过细胞膜从而在活细胞线粒体内聚集，并发出黄绿色荧光。广泛用作检测线粒体膜电位的荧光探针，并且对细胞没有任何毒性。 

  溶液的配制

  用DMSO溶解，终浓度为1-5 mM。溶液分装，避光保存于-20 ºC。

  罗丹明123使用方法

  1. 用载玻片准备细胞。细胞数目应为 5×104～5×105个/mL。

  2. 孵育该载玻片，用 PBS或Hank’s 液洗涤细胞。

  3. 用培养基稀释 Rh123母液以制备 1～20µM Rh123缓冲液。具体工作浓度取决于细胞类型和细胞浓度。

  4. 将 Rh123缓冲液加入载玻片并在37℃下孵育 30 分钟至 1 小时。

  5. 去除 Rh123 缓冲液并用培养基洗涤细胞（洗涤细胞后为了固定，加入 10%福尔马林缓冲液并孵育 15～20 分钟，接着用 PBS洗涤）。

  6. 用带有荧光素滤光片的荧光显微镜观察细胞。

  为了您的安全和健康，请穿实验服并戴一次性手套操作。

• 熔点： 235℃

• 溶解性： Soluble  in  ethanol  (20  mg/ml),  DMSO,  methanol,  and  water  (partly  miscible).

• 储存条件： RT

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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输入产品批号：

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *