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• 产品描述： Sivelestat is a potent and selective inhibitor of neutrophil elastase with IC50 of 44 nM.

• 靶点： SARS-CoV;Serine Protease;SARS-CoV;SerineProtease

• 体内研究：
  Administration of sivelestat attenuates pulmonary fibrosis after acute lung injury in mice. Sivelestat could attenuate sepsis-related kidney injury in rats. It blocks GAPDH/Siah1 which is a novel signaling pathway during the progression of spinal cord injury.

• 细胞实验： TMK-1 Gastric carcinoma cells are seeded into 96-well culture plates (5 ×103 cells/well) in RPMI containing 7% FBS, 2.0 mM L-glutamine, and 1% nonessential amino acids. Following 24-h incubation in RPMI-1640 medium with 5% FBS, the cells are incubated for 24, 48 or 72 h at 37°C in a humidified atmosphere of 5% CO2 with varied concentrations (0.1–1.0 μg/mL) of NE, or with varied concentrations (0.1–1000 μg/mL) of sivelestat or with concentrations (10 and 100 ng/mL) of anti-TGF-α antibody in the presence of 1 μg/mL NE. After incubation, 10 μL of MTT solution (5 mg/mL) is added to each well and the plates are then incubated for 3 h at 37°C. The growth medium is then replaced with 150 μL of dimethyl sulfoxide (Wako) per well and the absorbance at 540 nm is measured.

• 动物实验： Animal Models: Male Sprague-Dawley rats. Formulation: Saline. Dosages: 50 or 100 mg/kg. Administration: i.p.

• 参考文献：
  1.Li G, et al. Int J Mol Med. 2016, 38(3):767-75. 2.Huo J, et al. Neuroscience. 2016, 330:171-180. 3.Hagiwara S, et al. Inflamm Res. 2009, 58(4):198-203. 4.Wada Y, et al. Cancer Science. 2006, 97(10):1037-1043. 5.Araki Y, et al. Dig Dis Sci. 2011, 56(6):1672-81.

• 溶解性： Ethanol:80  mg/mL  (184.1  mM)    H2O:<1  mg/mL    DMSO:80  mg/mL  (184.1  mM)

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.302 ml	11.508 ml	23.017 ml
  5 mM	0.46 ml	2.302 ml	4.603 ml
  10 mM	0.23 ml	1.151 ml	2.302 ml
  50 mM	0.046 ml	0.23 ml	0.46 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *