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• 产品描述： Methylene Blue (Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015)在色素内镜检查中被作用染料。它能抑制tau蛋白丝的形成，IC50为1.9μM，同时还抑制可溶性鸟苷环化酶

• 靶点： guanylate cyclase; Tau aggregation:1.9 μM;Guanylatecyclase;MicrotubuleAssociated;NOSynthase;Parasite;MonoamineOxidase

• 体外研究：
  Methylene Blue可能抑制NO合成酶或通过产生超氧阴离子，直接使一氧化氮失活。除了干扰一氧化氮换鸟苷酸途径，Methylene Blue直接激活钙离子依赖性的钾通道来增强去甲肾上腺素从细胞内的释放

• 体内研究：
  Methylene Blue抑制鸟苷酸环化酶，减少C-GMP和血管平滑松弛。当进行静脉注射时，亚甲蓝在甲状旁腺积累，从而在外科手术中借以其颜色来判断这些内部结构。在啮齿类的药动学实验揭示，血管内进行MB给药将导致MB在中枢神经细胞中迅速广泛的积累

• 细胞实验： Cell lines: 未成熟的成神经细胞 Concentrations: 10 μM Incubation Time: 2 h Method: 从新生大鼠的室管膜下层分离出来的未成熟的成神经细胞，在单细胞培养模型中，分化成γ氨基丁酸所介导神经元，用此神经元细胞用于后续实验。在体外实验中，第6天，用MB对细胞培养物进行处理2h，处理完48小时后，检测细胞树突分枝结构及生存情况

• 动物实验： Animal Models: Sprague- Dawley雄性大鼠 Dosages: 5 and 50 mg/kg Administration: 血管内注射

• 参考文献：
  1. Vutskits L, et al. Adverse effects of methylene blue on the central nervous system. Anesthesiology. 108(4):684-692. 2. Hochgr鋐e K, et al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015, 3:25. 3. Masaki E, et al. Methylene blue, a soluble guanylyl cyclase inhibitor, reduces the sevoflurane minimum alveolar anesthetic concentration and decreases the brain cyclic guanosine monophosphate content in rats. Anesth Analg. 1999, 89(2):484-489. 4. Ginimuge PR, et al. Methylene blue: revisited. J Anaesthesiol Clin Pharmacol. 2010, 26(4):517-520.

• 溶解性： Soluble  in  DMSO、Ethanol、H2O

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	3.126 ml	15.632 ml	31.265 ml
  5 mM	0.625 ml	3.126 ml	6.253 ml
  10 mM	0.313 ml	1.563 ml	3.126 ml
  50 mM	0.063 ml	0.313 ml	0.625 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

• 24. [4.7] Tingyue Yu et al."Characteristics of Films Prepared from Wheat Gluten and Phenolic Extracts from Porphyra haitanensis and Its Application for Salmon Preservation."Foods.2024 Jan;13(15):2442

• 23. [IF=3.1] Shuyi Mi et al."HIF-1α/METTL1/m7G axis is involved in CRC response to hypoxia."BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2023 Dec;:149385

• 22. [IF=6] Han Ru et al."Cinnabaris modified with SiO2 nanoparticles stabilized Pickering emulsion to improve the photostability of volatile oil: A Lingzhu San case study."Arabian Journal of Chemistry.2024 Jan;17:105442

• 21. [IF=5.4] Bo Peng et al."Preparation and Characterization of an Oyster Peptide–Zinc Complex and Its Antiproliferative Activity on HepG2 Cells."Marine Drugs.2023 Oct;21(10):542

• 20. [IF=3.8] Yu Gao et al."Evaluation of topical methylene blue nanoemulsion for wound healing in diabetic mice."PHARMACEUTICAL BIOLOGY.10.1080/13880209.2023.2254341

• 19. [IF=4.8] Mengjie Li et al."Sensitive photoelectrochemical detection of Hg2+ with low background noise."MICROCHEMICAL JOURNAL.10.1016/j.microc.2023.109429

• 18. [IF=9.9] Hanzhu Wu et al."Fabrication of antifouling UiO-66 nanofiltration membranes via surface fluorination engineering."DESALINATION.10.1016/j.desal.2023.116957

• 17. [IF=8.2] Feng Cao et al."Preparation, characterization and in vitro digestion of octenyl succinic anhydride-modified porous starch with different degrees of substitution."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2023 Dec;253:126579

• 16. [IF=2.7] Xiao-Hui Zhang et al."Synthesis of CuO/g-C3N4 Composite with High Anti-Interference Ability for Enhanced Fenton-Like Catalysis."PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION.2023 Jul;:2300035

• 15. [3.748] Qian Wei et al."MoS2/Au Heterojunction Catalyst for SERS Monitoring of a Fenton-like Reaction."Materials.2023 Jan;16(3):1169

• 14. [7.968] Zhanyao Li et al."Research on the hydrothermal regeneration of powdered activated coke in wastewater treatment."Journal of Environmental Chemical Engineering.2022 Dec;:109120

• 13. [IF=3.898] Yujia Cui et al."One-step crosslinking preparation of tannic acid particles for the adsorption and separation of cationic dyes."CHINESE JOURNAL OF CHEMICAL ENGINEERING.2022 Aug;:

• 12. [IF=5.518] Man-Ke Zhang et al."A novel alginate/PVA hydrogel -supported Fe3O4 particles for efficient heterogeneous Fenton degradation of organic dyes."COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS.2022 Nov;652:129830

• 11. [IF=7.348] Yi Feng et al."Cascade-activatable NO release based on GSH-detonated “nanobomb” for multi-pathways cancer therapy."Materials Today Bio. 2022 Mar;14:100288

• 10. [IF=3.196] Peng Cao et al."Complecting the BiOCl nano-roundels based hollow microbasket induced by chitosan for dramatically enhancing photocatalytic activity."J Mol Struct. 2022 Apr;1254:132339

• 9. [IF=9.229] Xin Wang et al."Low-Cost, Robust Pressure-Responsive Smart Windows with Dynamic Switchable Transmittance."Acs Appl Mater Inter. 2020;12(13):15695–15702

• 8. [IF=12.479] Yi Feng et al."Multistage-responsive nanovehicle to improve tumor penetration for dual-modality imaging-guided photodynamic-immunotherapy."Biomaterials. 2021 Aug;275:120990

• 7. [IF=13.273] Baoshan He et al."Nb.BbvCI powered DNA walking machine-based Zr-MOFs-labeled electrochemical aptasensor using Pt@AuNRs/Fe-MOFs/PEI-rGO as electrode modification material for patulin detection."Chem Eng J. 2021 Feb;405:126642

• 6. [IF=1.614] Chenghui Zheng et al."Microwave-assisted preparation of pyrite and its sensitisation of titanium dioxide in self-cleaning aramid fabrics."Color Technol. 2018 Aug;134(4):284-291

• 5. [IF=3.361] Jieru Lin et al."Surface-enhanced Raman spectroscopic identification in fingerprints based on adhesive Au nanofilm."Rsc Adv. 2018 Jul;8(43):24477-24484

• 4. Baoshan He, Xiaoze Dong, Nb.BbvCI powered DNA walking machine-based Zr-MOFs-labeled electrochemical aptasensor using Pt@AuNRs/Fe-MOFs/PEI-rGO as electrode modification material for patulin detection, Chemical Engineering Journal, Volume 405, 2021, 126642,

• 3. Wu J, Xia A, Chen C, Feng L, Su X, Wang X. Adsorption Thermodynamics and Dynamics of Three Typical Dyes onto Bio-adsorbent Spent Substrate of Pleurotus eryngii. International Journal of Environmental Research and Public Health. 2019; 16(5):679. https://doi

• 2. Liu, Rong, et al. "Direct and comprehensive analysis of dyes based on integrated molecular and structural information via laser desorption laser postionization mass spectrometry." Talanta 176 (2018): 116-123.https://doi.org/10.1016/j.talanta.2017.07.069

• 1. Chenghui Zheng, Zhenming Qi & Guoqiang Chen (2016) Silver sulfide quantum dots as sensitizer in self-cleaning of Bombyx mori silk fabrics with nano-titania, The Journal of The Textile Institute, 107:12, 1501-1510, DOI: 10.1080/00405000.2015.1128227

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *