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• 产品描述： TTNPB, a potent RAR agonist, inhibits binding of [3H]tRA of human RARα (IC50: 5.1 nM), β (IC50: 4.5 nM), and γ (IC50: 9.3 nM), respectively.

• 靶点： Apoptosis;Autophagy;Retinoid Receptor

• 体内研究：
  在条件培养基培养72 h,TTNPB可使JEG-3细胞中小鼠mRARα（EC50：2.0 nM）、β（EC50：1.1 nM）以及γ（EC50：0.8 nM）转录活性增加。TTNPB与核视黄酸受体的结合亲和力较高,从而抑制[3H]tRA与mRARα（IC50：3.8 nM）、 β（IC50：4.0 nM）以及γ（IC50：4.5 nM）的结合。通过诱导G1细胞周期阻滞,TTNPB对雌激素受体-阳性乳腺癌细胞和正常人体乳腺上皮细胞的生长具有抑制作用。TTNPB浓度依赖性地减少ES-D3细胞分化

• 细胞实验： Human mammary epithelial cells are maintained in Mammary Epithelial Basal Medium (MEBM) supplemented with the Mammary Epithelial Growth Media (MEGM) bullet kit. 184 and 184B5 cells are maintained in MEBM sodium-bicarbonate free (MEBM-SBF) supplemented with the MEGM bullet kit, isoproterenol (10 μM), and transferrin (5 μg/ml). MCF10A cell lines are maintained in DME/F12 containing 5% heat inactivated horse serum, penicillin/streptomycin (100 μg/ml and 100 μg/ml), hydrocortisone (1.4 μM), insulin (10 μg/ml), choleratoxin (100 ng/ml), and EGF (20 ng/ml). Breast cancer cell lines are maintained in Improved MEM Zinc Option containing 10% fetal bovine serum, 1% glutamine, and 1% penicillin/streptomycin. For growth assays, cells are treated with the different retinoids for the specified number of days with media and treatment changes every other day in T47D cells and every 2 days in 184 cells. Cell proliferation is measured according to the protocol for the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Ass

• 参考文献：
  1.Darro F, et al. Breast Cancer Res Treat. 1998, 51(1), 39-55. 2.Pignatello MA, et al. Toxicol Appl Pharmacol. 1999, 159(2), 109-116. 3.Wu K, et al. Breast Cancer Res Treat. 2006, 96(2), 147-157. 4.Louisse J, et al. Toxicol Lett. 2011, 203(1), 1-8. 5.Pignatello MA, et al. Toxicol Appl Pharmacol. 1997, 142(2), 319-327.

• 溶解性： DMSO:3.5  mg/mL  (10  mM)

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.87 ml	14.348 ml	28.696 ml
  5 mM	0.574 ml	2.87 ml	5.739 ml
  10 mM	0.287 ml	1.435 ml	2.87 ml
  50 mM	0.057 ml	0.287 ml	0.574 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *