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• 产品描述： AP20187 (B/B Homodimerizer, HY-13992) 是一种二聚化的化学诱导剂，可激活 FKBP-Casp8

• 靶点： FKBP; Casp8;Others

• 体外研究：
  In oligodendrocytes, AP20187 treatment increases the expression of PERK-responsive genes encoding CAATT enhancer binding protein homologous protein (CHOP) and growth arrest and DNA damage 34 (GADD34) in a dose-dependent manner. The activity of PERK signaling in oligodendrocytes can be temporally manipulated by the administration of AP20187

• 体内研究：
  AP20187 treatment significantly reduces the number of degenerating axons and increases the density of axons in the demyelinating lesions in the lumbar spinal cord of PLP/Fv2E-PERK mice. AP20187 is also sufficient to activate Fv2E-PERK in oligodendrocytes in the CNS of PLP/Fv2E-PERK mice.

• 细胞实验： Cell lines: Oligodendrocytes Concentrations: 1 nM Incubation Time: 4 h Method: After 3 d of differentiation, the cells are treated with 1 nM AP20187 or vehicle for 4 h and are then fixed with cold 4% paraformaldehyde in PBS. The cells are blocked with PBS containing 10% goat serum and 0.1% Triton X-100 and incubate overnight with the primary antibody diluted in blocking solution. Appropriate fluorochrome-labeled secondary antibodies are used for detection.

• 动物实验： Animal Models: PLP/Fv2E-PERK mice Dosages: 0.5 mg/kg, 2 mg/kg, 5 mg/kg Administration: i.p.

• 参考文献：
  1. Darren J Baker, et al. Naturally occurring p16(Ink4a)-positive cells shorten healthy lifespan. Nature . 2016 Feb 11;530(7589):184-9. 2. Wensheng Lin, et al. Oligodendrocyte-specific activation of PERK signaling protects mice against experimental autoimmune encephalomyelitis. J Neurosci . 2013 Apr 3;33(14):5980-91.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	0.674 ml	3.372 ml	6.744 ml
  5 mM	0.135 ml	0.674 ml	1.349 ml
  10 mM	0.067 ml	0.337 ml	0.674 ml
  50 mM	0.013 ml	0.067 ml	0.135 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *