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• 产品描述： TCS 401 is a selective inhibitor of protein-tyrosine phosphatase 1B (PTP1B; Ki = 0.29 μM)

• 靶点： Phosphatase

• 体外研究：
  TCS 401 promoted the proliferation and expression of cyclin A and cyclin D1 in RPE cells. TCS 401 induced RPE cells to differentiate toward better contractility and motility. A migration assay proved that inhibiting PTP1B improved the migratory activity of RPE cells. TCS 401 activated extracellular signal-regulated kinase (Erk) and protein kinase B (Akt) phosphorylation. Pretreatment with PD98059 and LY294002 abolished TCS 401-induced activation of Erk, Akt, cell proliferation, and cell migration

• 细胞实验： From the cultured RPE cells treated with TCS 401, cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetracolium bromide assay, and the protein expression levels of cyclin A and cyclin D1 were determined. The effect of TCS 401 on cell differentiation was confirmed by immunostaining for α-smooth muscle actin and by western blot. Cell migration activity and PTP1B signaling mechanism were determined. Migration Assay was used to evaluate cell migration activity. PTP1B signaling mechanism was determined by use of PD98059 and LY294002.

• 参考文献：
  1. Du Z D , Hu L T , Zhao G Q , et al. Protein tyrosine phosphatase 1B regulates the activity of retinal pigment epithelial cells[J]. Molecular vision, 2015, 21:523-531. 2. Necrotic renal epithelial cell inhibits renal interstitial fibroblast activation: role of protein tyrosine phosphatase 1B.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	3.26 ml	16.301 ml	32.603 ml
  5 mM	0.652 ml	3.26 ml	6.521 ml
  10 mM	0.326 ml	1.63 ml	3.26 ml
  50 mM	0.065 ml	0.326 ml	0.652 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *