| 产品描述： | TCS 401 is a selective inhibitor of protein-tyrosine phosphatase 1B (PTP1B; Ki = 0.29 μM) |
| 靶点： |
Phosphatase |
| 体外研究： |
TCS 401 promoted the proliferation and expression of cyclin A and cyclin D1 in RPE cells. TCS 401 induced RPE cells to differentiate toward better contractility and motility. A migration assay proved that inhibiting PTP1B improved the migratory activity of RPE cells. TCS 401 activated extracellular signal-regulated kinase (Erk) and protein kinase B (Akt) phosphorylation. Pretreatment with PD98059 and LY294002 abolished TCS 401-induced activation of Erk, Akt, cell proliferation, and cell migration |
| 细胞实验： |
From the cultured RPE cells treated with TCS 401, cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetracolium bromide assay, and the protein expression levels of cyclin A and cyclin D1 were determined. The effect of TCS 401 on cell differentiation was confirmed by immunostaining for α-smooth muscle actin and by western blot. Cell migration activity and PTP1B signaling mechanism were determined. Migration Assay was used to evaluate cell migration activity. PTP1B signaling mechanism was determined by use of PD98059 and LY294002. |
| 参考文献： |
1. Du Z D , Hu L T , Zhao G Q , et al. Protein tyrosine phosphatase 1B regulates the activity of retinal pigment epithelial cells[J]. Molecular vision, 2015, 21:523-531. 2. Necrotic renal epithelial cell inhibits renal interstitial fibroblast activation: role of protein tyrosine phosphatase 1B. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-20℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
3.26 ml |
16.301 ml |
32.603 ml |
| 5 mM |
0.652 ml |
3.26 ml |
6.521 ml |
| 10 mM |
0.326 ml |
1.63 ml |
3.26 ml |
| 50 mM |
0.065 ml |
0.326 ml |
0.652 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京