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• 产品描述： Alda 1是一种ALDH2激动剂，能激活野生型ALDH2 (ALDH2*1)和ALDH2*2的催化活性

• 靶点： ALDH2;Apoptosis;Dehydrogenase

• 体外研究：
  Alda-1 alleviated liver injury after hepatic I/R. Pretreatment with Alda-1 significantly alleviated I/R-induced elevations of alanine aminotransferase and aspartate aminotransferase, and significantly blunted the pathological injury of the liver. Moreover, Alda-1 significantly inhibited ROS and proinflammatory cytokines production, 4-HNE and MDA accumulation, and apoptosis. Increased ALDH2 activity was found after Alda-1 administration. No significant changes in ALDH2 expression were observed after I/R

• 细胞实验： Cell lines: normal rat hepatocyte cell (BRL-3A) Concentrations: 20 μM Incubation Time: 1 hour Method: The H/R (Hypoxia/reoxygenation) group cells are incubated under a hypoxic atmosphere (95% N2+5% CO2) for 6 h followed by 120 min reoxygenation (95% air + 5% CO2). The control incubation is normoxic conditions (95% air + 5% CO2) for the entire experiment. In the H/R+Alda-1 group, cells are incubated with Alda-1 (20 μM) for 60 min at 37℃ (95% air/5% CO2) before exposure to hypoxia. Furthermore, BRL-3A cells are treated with 5 μM 4-HNE for 60min before exposure to hypoxia to estimate whether 4-HNE could affect H/R.

• 动物实验： Animal Models: Male Sprague-Dawley rats (IR model) Dosages: 10 mg/kg Administration: i.p.

• 参考文献：
  1. Samantha Perez-Miller, et al. Alda-1 is an agonist and chemical chaperone for the common human aldehyde dehydrogenase 2 variant. Nat Struct Mol Biol. 2010, 17(2): 159-164. 2. Zhang T, et al. Alda-1, an ALDH2 activator, protects against hepatic ischemia/reperfusion injury in rats via inhibition of oxidative stress. Free Radic Res. 2018, 52(6):629-638.

• 溶解性： Soluble  in  DMSO

• 保存条件： 2-8℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	3.085 ml	15.424 ml	30.849 ml
  5 mM	0.617 ml	3.085 ml	6.17 ml
  10 mM	0.308 ml	1.542 ml	3.085 ml
  50 mM	0.062 ml	0.308 ml	0.617 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *