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• 产品描述： Skp2 Inhibitor C1 (SKPin C1) is an S-phase kinase-related protein 2 (Skp2) inhibitor with an inhibitory effect on metastatic melanoma cells. Skp2 Inhibitor C1 slows the cell cycle, inhibits cell proliferation, and triggers apoptosis

• 靶点： E1/E2/E3 Enzyme;E1/E2/E3Enzyme

• 体外研究：
  Skp2 Inhibitor C1 (10-50 μM; 12 hr) decreases the viability of THP-1, U266 and RPMI 8226 cells. Skp2 Inhibitor C1 (25 μM) increases p27 protein levels in U266 and RPMI 8226 cells by inhibiting ubiquitination. Skp2 Inhibitor C1 (25 μM) inhibits cell cycle of U266 and RPMI 8226 cells. Cell Cycle Analysis Cell Line: B lymphocytes, THP-1, U266 and RPMI 8226 cells Concentration: 0, 5, 10, 25, and 50 μM Incubation Time: 12 hr, 24 hr, 36 hr, and 48 hr Result: Significantly decreased the viability of U266 and RPMI 8226 cells at 10 μM for 12 hours.Did not significantly affect B lymphocyte viability at 50 μM.Decreased THP-1 cell viability at 50 μM for 12 hours. Cell Viability Assay Cell Line: U266 and RPMI 8226 cells Concentration: 0, 5, 10, 25, and 50 μM Incubation Time: 12 hr Result: Increased the percentages of U266 and RPMI 8226 cells in the G0/G1 phase, while decreased the percentages in S and G2/M phases.

• 体内研究：
  Skp2 Inhibitor C1 (5 mg/kg and 10 mg/kg; 3 times within 24 h: 24, 5, and 1 h before the test) shows the antidepressant-like effect in mouse models following chronic treatment by using the tail suspension test (TST), forced swimming test (FST), and social interaction test (SIT)

• 参考文献：
  1. Yang Y, et al. Skp2 inhibitor SKPin C1 decreased viability and proliferation of multiple myeloma cells and induced apoptosis. Braz J Med Biol Res. 2019;52(5):e8412. 2. Li F, et al. Identification of the antidepressive properties of C1, a specific inhibitor of Skp2, in mice. Behav Pharmacol. 2021 Feb 1;32(1):62-72.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.149 ml	10.745 ml	21.49 ml
  5 mM	0.43 ml	2.149 ml	4.298 ml
  10 mM	0.215 ml	1.074 ml	2.149 ml
  50 mM	0.043 ml	0.215 ml	0.43 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *