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• 产品描述： Angiotensin 1-7, also known as Ang-(1-7), is a synthetic heptapeptide identical to endogenous angiotensin-(1-7) , inhibits purified canine ACE activity (IC50=0.65 μM) with vasodilator and antiproliferative activities.

• 靶点： Endogenous Metabolite;RAAS;RAAS;EndogenousMetabolite

• 体内研究：
  Ang-(1-7) treatment attenuated the shortening of the atrial effective refractory period and the increase in the atrial fibrillation inducibility level. Immunohistochemistry and Western blotting showed sympathetic nerve hyperinnervation in the pacing group, while Ang-(1-7) attenuated sympathetic nerve proliferation. Ang-(1-7) alleviated the pacing-induced increases in tyrosine hydroxylase and nerve growth factor mRNA expression levels

• 细胞实验： Cultured cardiomyocytes were treated with PBS (control), ANG II (100 nM), ANG-(1–7) (100 nM), ANG II + ANG-(1–7), ANG II + ANG-(1–7) + A779 (10 μM), ANG II + ANG-(1–7) + Sirt3 short hairpin (sh)RNA, lentiviral particles (1 × 10^6 TU) containing a Sirt3 shRNA viral vector, or ANG II + Mito-TEMPO (0.1 μM) for 24 h. Each treatment was performed in triplicate wells. Monoclonal anti-α-sarcomeric actin antibody was used for identification and measurement of cardiomyocytes via immunofluorescence staining. Cells were fixed in methanol at 10°C and washed with fresh washing solution (0.1% Triton X-100 in PBS) three times. After preincubation with 3% BSA for 20 min, cells were incubated with primary antibody, which was diluted to 1:100 with PBS containing 3% BSA, overnight at 4°C. After three washings with the washing solution, cells were incubated with fluorescence-conjugated anti-mouse IgG (1:500 diluted in PBS) for 1 h at room temperature. Photographic images of cardiomyocytes were taken with a fluorescence microscop

• 动物实验： Eighteen dogs were randomly assigned to sham group, pacing group and Ang-(1-7) group. Rapid atrial pacing was maintained for 14 days in the pacing and Ang-(1-7) groups. Ang-(1-7) was administered intravenously in the Ang-(1-7) group. The atrial effective refractory period and atrial fibrillation inducibility level were measured at baseline and under sympathetic nerve stimulation after 14 days of measurement. The atrial sympathetic nerves labeled with tyrosine hydroxylase were detected using immunohistochemistry and Western blotting, and tyrosine hydroxylase and nerve growth factor mRNA levels were measured by reverse transcription polymerase chain reaction

• 参考文献：
  1.Guo L , Yin A , Zhang Q , et al. Angiotensin (1-7) Attenuates Angiotensin II-induced Cardiac Hypertrophy via a Sirt3-Dependent Mechanism[J]. American Journal of Physiology - Heart and Circulatory Physiology, 2017:ajpheart.00768.2016. 2.Shangguan W , Shi W , Li G , et al. Angiotensin-(1–7) attenuates atrial tachycardia-induced sympathetic nerve remodeling[J]. Journal of the Renin-Angiotensin-Aldosterone System, 2017, 18(3):147032031772928.

• 溶解性： Soluble  in  H2O

• 保存条件： -20°C

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	1.112 ml	5.562 ml	11.123 ml
  5 mM	0.222 ml	1.112 ml	2.225 ml
  10 mM	0.111 ml	0.556 ml	1.112 ml
  50 mM	0.022 ml	0.111 ml	0.222 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *