• 产品描述： QX77 is a chaperone-mediated autophagy (CMA) activator and upregulates LAMP2A expression in vitro. QX77 induces Rab11 upregulation, rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 can impede self-renewal and promote differentiation of ES cells

• 靶点： CMA;Autophagy

• 体外研究：
  QX77 (48 hours) upregulates Rab11 expression levels in Ctns-/- MEFs. In Ctns-/- MEFs, QX77 recovers the down-regulated LAMP2A expression, and the Rab11-positive carrier vesicles recover the high-motility trafficking phenotype observe in wild-type cells. Treatment with CMA activator QX77 rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 treatment also increases LAMP2A localization at the lysosomal membrane. QX77 significantly increases the re-localization of LAMP2A at LAMP1-positive lysosomes in cystinotic cell, it corrects the localization of LAMP2A at the lysosomal membrane in cystinotic cells. QX77 protects cystinotic cells from the increased susceptibility to tert-butyl-hydroperoxide-induced oxidative stress and reconstitutes the resistant levels observed in wild-type cells. The effect of QX77 on cystinotic cell survival is dependent on LAMP2A expression. QX77 (10 μM; 0, 3 or 6 days) activates CMA and increases LAMP2A expression in D3 and E14 ES cell, it downregulates pluripotency factors and AP reactivity and partially lost the characteristic ES cell morphology. Western Blot Analysis Cell Line: ES D3 cell line; E14TG2a (E14) cell line Concentration: 10 μM Incubation Time: 3 or 6 days Result: Increased LAMP2A expression and decreased SOX2 and Oct4 protein expression.

• 参考文献：
  1. Jinzhong Zhang, et al. Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A. J Biol Chem. 2017 Jun 23;292(25):10328-10346 2. Yi Xu, et al. Chaperone-mediated autophagy regulates the pluripotency of embryonic stem cells. Science. 2020 Jul 24;369(6502):397-403.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	3.325 ml	16.626 ml	33.251 ml
  5 mM	0.665 ml	3.325 ml	6.65 ml
  10 mM	0.333 ml	1.663 ml	3.325 ml
  50 mM	0.067 ml	0.333 ml	0.665 ml

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• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *