• 产品描述： ML367 是一种有效的 ATPase family AAA domain-containing protein 5 (ATAD5) 的微摩尔级抑制剂。ML367 会阻断 DNA repair 途径和一般的 DNA damage 反应，包括紫外线刺激的RPA32磷酸化和CHK1磷酸化

• 靶点： ATAD5;EpigeneticReaderDomain

• 体外研究：
  ML367 is a probe molecule found to block general DNA damage responses including RPA32-phosphorylation and CHK1-phosphorylation in response to UV irradiation. In this regard, the probe molecule could block DNA repair pathways that function upstream of ATAD5. Additionally, ML367 sensitizes cells possessing a knock-out mutation of the PARP1 gene and as a result may serve as a sensitizer to kill cancer cells defective in the poly (ADP-ribose) polymerase 1 (PARP1)-dependent DNA repair pathway.

• 细胞实验： Cell lines: HEK293T cells Concentrations: 5, 10, 20 and 40 μM Incubation Time: 16 h Method: HEK293T cells were transiently transfected with FLAG-tagged ATAD5 and treated with the indicated amount of ML367 for 16 hours in the presence or absence of 20 μM 5-FUrd. ATAD5 protein levels were visualized by western blotting using an antibody against FLAG, and quantified using ImageJ.

• 参考文献：
  1. Jason M. Rohde, et al. Discovery of ML367, inhibitor of ATAD5 stabilization. Probe Reports from the NIH Molecular Libraries Program. Bethesda: National Center for Biotechnology Information (US); 2010–.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.991 ml	14.956 ml	29.911 ml
  5 mM	0.598 ml	2.991 ml	5.982 ml
  10 mM	0.299 ml	1.496 ml	2.991 ml
  50 mM	0.06 ml	0.299 ml	0.598 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *