| 产品描述： | QStatin, a Selective Inhibitor of Quorum Sensing in Vibrio Species( SmcR with an EC50 of 208.9 nM). |
| 靶点： |
Antibacterial |
| 体外研究： |
Because 377B6 [1-(5-bromothiophene-2-sulfonyl)-1H-pyrazole](QStatin) showed the strongest inhibition of SmcR activity without significant growth attenuation of V. vulnificus. SmcR activity was assessed by measuring the RLU level for V. vulnificus WT (pBB1) in the presence of different concentrations of QStatin, which revealed that the half-maximal effective concentration (EC50) of QStatin was 208.9 nM. |
| 细胞实验： |
The plasmid pBB1 carrying the LuxRVh homologue-activated lux operon was conjugally transferred into the V. vulnificus wild-type (WT) strain, the ΔsmcR mutant, or the ΔluxO mutant. These V. vulnificus reporter strains were grown overnight, diluted 1:1,000 in fresh LBS, and treated with hit molecules. RLU values were calculated every hour. To determine the EC50, QStatin (10^12 to 10^4 M) or 2% DMSO as a control was added to the V. vulnificus WT reporter strain and RLU was measured after 5 h. The percentage of SmcR activity of the sample at a given concentration of QStatin was determined using the following equation: percent SmcR activity = sample RLU/control RLU × 100. The EC50 of QStatin (the concentration reducing the SmcR activity to 50%) was calculated from a plot of the percentages of SmcR activity versus QStatin concentrations using GraphPad Prism 6.0. |
| 参考文献： |
1. Kim B S , Jang S Y , Bang Y J , et al. QStatin, a Selective Inhibitor of Quorum Sensing in Vibrio Species[J]. mBio, 2018, 9(1). |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
2-8℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
3.411 ml |
17.056 ml |
34.111 ml |
| 5 mM |
0.682 ml |
3.411 ml |
6.822 ml |
| 10 mM |
0.341 ml |
1.706 ml |
3.411 ml |
| 50 mM |
0.068 ml |
0.341 ml |
0.682 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京