• 产品描述： SPI-112 是一种非细胞渗透的具有 Src homology region 2 domain-containing phosphatase 2 (SHP-2, PTPN11) 的抑制剂，在无细胞试验中IC50值为1 μM

• 靶点： SHP-2(Cell-free assay):1 μM;Phosphatase

• 体外研究：
  SPI-112 is a not cell permeable inhibitor of Src homology region 2 domain-containing phosphatase 2 (SHP-2, PTPN11) with IC50 of 1.0 μM, suggesting that SPI-112 binds to the catalytic pocket

• 细胞实验： Cell lines: MDA-MB-468 breast cancer cells Concentrations: 100 μM Incubation Time: 10 min Method: Sub-confluent MDA-MB-468 cells were serum-starved in DMEM/0.1% BSA for 18 h, pretreated overnight with SPI-112 (100 μM) and stimulated with EGF (50 ng/ml, 10 min) or mocked treated as indicated. Shp2 was immunoprecipitated from cell lysate supernatants and Shp2 PTP activity was measured.

• 参考文献：
  1. Liwei Chen, et al. Inhibition of cellular Shp2 activity by a methyl ester analog of SPI-112. Biochem Pharmacol. 2010 Sep 15;80(6):801-10.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.135 ml	10.673 ml	21.347 ml
  5 mM	0.427 ml	2.135 ml	4.269 ml
  10 mM	0.213 ml	1.067 ml	2.135 ml
  50 mM	0.043 ml	0.213 ml	0.427 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

• 1. [IF=5.354] Anlei Ge et al.Alteration in rheology and microstructure of O/W emulsions using controlled soy protein isolate-polysaccharide aggregation in aqueous phases.J Food Eng. 2022 Mar;317:110872

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *