| 产品描述： | 4-Octyl Itaconate激活Nrf2信号，在人类巨噬细胞和分离自全身性红斑狼疮患者的PBMC中抑制促炎性因子的产生 |
| 靶点： |
Nrf2 |
| 体外研究： |
4-Octyl Itaconate effectively prevents NP cells from degeneration through anti-ROS and anti-inflammation, and the combined use with tBHQ has better effects |
| 体内研究： |
4-Octyl itaconate is a crucial anti-inflammatory metabolite that acts via Nrf2 to limit inflammation and modulate type I interferons |
| 细胞实验： |
Cell lines: nucleus pulposus (NP) cells Concentrations: 5, 10, 15, 20, 25, 30 µM Incubation Time: 24 h Method: The NP cells are cultured in the 24 well plates with a density of 1.5×104 per well. To test the optimized concentration of tBHQ, 4-Octyl Itaconate, and the mixture of them, ranged concentration of drugs is used to stimulate NP cells for 24 hours. The highest concentration that did not affect the cell viability is applied in our experiment. Besides, we also used Lipopolysaccharides to induce NP cell degeneration. |
| 动物实验： |
Animal Models: C57BL/6 mice Dosages: 50 mg/kg Administration: i.p. |
| 参考文献： |
1. Zhong WX, et al. The effect of Nrf2 activators tBHQ and 4-octyl itaconate on the nucleus pulposus cell degeneration. Eur Rev Med Pharmacol Sci. 2021 Aug;25(16):5189-5198. 2. Mills EL, et al. Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1. Nature. 2018 Apr 5;556(7699):113-117. |
| 溶解性： |
Soluble in DMSO、Ethanol |
| 保存条件： |
-20°C |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
4.127 ml |
20.635 ml |
41.269 ml |
| 5 mM |
0.825 ml |
4.127 ml |
8.254 ml |
| 10 mM |
0.413 ml |
2.063 ml |
4.127 ml |
| 50 mM |
0.083 ml |
0.413 ml |
0.825 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京