• 产品描述： 4-Octyl Itaconate激活Nrf2信号，在人类巨噬细胞和分离自全身性红斑狼疮患者的PBMC中抑制促炎性因子的产生

• 靶点： Nrf2

• 体外研究：
  4-Octyl Itaconate effectively prevents NP cells from degeneration through anti-ROS and anti-inflammation, and the combined use with tBHQ has better effects

• 体内研究：
  4-Octyl itaconate is a crucial anti-inflammatory metabolite that acts via Nrf2 to limit inflammation and modulate type I interferons

• 细胞实验： Cell lines: nucleus pulposus (NP) cells Concentrations: 5, 10, 15, 20, 25, 30 µM Incubation Time: 24 h Method: The NP cells are cultured in the 24 well plates with a density of 1.5×104 per well. To test the optimized concentration of tBHQ, 4-Octyl Itaconate, and the mixture of them, ranged concentration of drugs is used to stimulate NP cells for 24 hours. The highest concentration that did not affect the cell viability is applied in our experiment. Besides, we also used Lipopolysaccharides to induce NP cell degeneration.

• 动物实验： Animal Models: C57BL/6 mice Dosages: 50 mg/kg Administration: i.p.

• 参考文献：
  1. Zhong WX, et al. The effect of Nrf2 activators tBHQ and 4-octyl itaconate on the nucleus pulposus cell degeneration. Eur Rev Med Pharmacol Sci. 2021 Aug;25(16):5189-5198. 2. Mills EL, et al. Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1. Nature. 2018 Apr 5;556(7699):113-117.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20°C

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	4.127 ml	20.635 ml	41.269 ml
  5 mM	0.825 ml	4.127 ml	8.254 ml
  10 mM	0.413 ml	2.063 ml	4.127 ml
  50 mM	0.083 ml	0.413 ml	0.825 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

• 3. [IF=5.8] Zhu Li et al."Acod1/itaconate activates Nrf2 in pulmonary microvascular endothelial cells to protect against the obesity-induced pulmonary microvascular endotheliopathy."RESPIRATORY RESEARCH".2024 Dec;25(1):1-13

• 2. [IF=5.1] Ni Si-tao et al."Anti-Necroptotic Effects of Itaconate and its Derivatives."INFLAMMATION.10.1007/s10753-023-01909-z

• 1. [IF=3.333] Guoqing Sun et al."Itaconate Attenuates Neuroinflammation and Exerts Dopamine Neuroprotection in Parkinson’s Disease through Inhibiting NLRP3 Inflammasome."Brain Sciences.2022 Sep;12(9):1255

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *