• 产品描述： ISRIB 是一种 integrated stress response (ISR) 抑制剂，可有效逆转 eukaryotic initiation factor 2α (eIF2α) 磷酸化的作用，其IC50值为5 nM

• 靶点： ISR; eIF2α;PERK

• 体外研究：
  ISRIB, an integrated stress response (ISR) inhibitor, reverses the effect of thapsigargin (TG) on translation, notably inhibits ER stress-induced cFLIP loss and prevents cFLIP levels down-regulation

• 体内研究：
  In an instability-induced intervertebral disc degeneration (IDD) model in mice, ISRIB suppressed p-eIF2α/ATF4/Ihh and prevented IDD via protecting the anti-oxidative enzymes and decreased the NP cell apoptosis

• 细胞实验： Cell lines: HCT116 cells Concentrations: 220 nM Incubation Time: 1 h Method: HCT116 cells are pre-treated with ISRIB (220 nM) for 1 h prior TG treatment for the indicated times. To analyze protein synthesis, puromycin is added in the last 10 min of treatment and puromycin incorporation to the nascent protein chain is assessed by western blotting using an anti-puromycin antibody. Levels of both cFLIP isoforms are determined in whole cell extracts by western blotting and quantified

• 动物实验： Animal Models: C57 background BL/6J mice of intervertebral disc degeneration (IDD) models Dosages: 0.25 mg/kg Administration: s.c.

• 参考文献：
  1. Carmela Sidrauski, et al. Pharmacological brake-release of mRNA translation enhances cognitive memory. Elife. 2013 May 28;2:e00498. 2. Mora-Molina R, et al. cFLIP downregulation is an early event required for endoplasmic reticulum stress-induced apoptosis in tumor cells. Cell Death Dis. 2022;13(2):111. 3. Bao J, et al. Pharmacological Disruption of Phosphorylated Eukaryotic Initiation Factor-2α/Activating Transcription Factor 4/Indian Hedgehog Protects Intervertebral Disc Degeneration via Reducing the Reactive Oxygen Species and Apoptosis of Nucleus Pulposus Cells. Front Cell Dev Biol. 2021 Jun 7;9:675486.

• 溶解性： Soluble  in  DMSO

• 保存条件： 2-8°C

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.216 ml	11.078 ml	22.156 ml
  5 mM	0.443 ml	2.216 ml	4.431 ml
  10 mM	0.222 ml	1.108 ml	2.216 ml
  50 mM	0.044 ml	0.222 ml	0.443 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *