- 产品描述: J14 is a reversible sulfiredoxin inhibitor with an IC50 of 8.1 μM. J14 induces oxidative stress (intracellular ROS accumulation) by inhibiting sulfiredoxin, leading to cytotoxicity and cancer cell death
- 靶点: IC50: 8.1 μM (Sulfiredoxin); ROS;ReactiveOxygenSpecies
- 体外研究:
J14 (0-100 μM;0-96 hours;A549细胞) 处理对A549细胞的生长具有浓度和时间依赖性抑制作用,其对A549细胞生长的半抑制浓度为15.7 μM。 J14 (20 μM;48-72 小时;A549 细胞) 处理不仅会导致细胞色素 c 释放到细胞质中,还会激活 caspase-3 和 caspase-9 . J14 诱导线粒体氧化损伤,导致半胱天冬酶介导的细胞凋亡。 J14 处理显著增加亚磺酸过氧化物酶和细胞内 ROS 的积累。细胞内 ROS 的过量积累导致氧化损伤,导致细胞死亡。J14 以时间依赖性方式显著诱导 A549 细胞死亡,导致 96 小时内约 40% 的细胞死亡。 J14 诱导线粒体氧化损伤和细胞凋亡。 Cell Viability Assay Cell Line: A549 cells Concentration: 0-100 μM Incubation Time: 0 hour, 24 hours, 48 hours, 72 hours, 96 hours Result: Inhibited the growth of A549 cells in a concentration- and a time- dependent manner. Western Blot Analysis Cell Line: A549 cells Concentration: 20 μM Incubation Time: 48 hours, 72 hours Result: Caused not only the release of cytochrome c into the cytosol, but also the activation of caspase-3 and caspase-9.
- 体内研究:
J14 (50 mg/kg;腹膜内注射;每天;持续 16 天;BALB/c 雌性裸鼠) 处理显著降低了平均肿瘤体积。与对照小鼠相比,J14 处理小鼠的原发性肿瘤的质量和重量显著降低。 Animal Model: Six-week-old BALB/c nude female mice injected with A549 cells Dosage: 50 mg/kg Administration: Intraperitoneal injection; daily; for 16 days Result: Significantly reduced the growth of human lung tumor without acute toxicity.
- 参考文献:
1. Kim H, et al. Sulfiredoxin inhibitor induces preferential death of cancer cells through reactive oxygen species-mediated mitochondrial damage. Free Radic Biol Med. 2016 Feb;91:264-74.
- 溶解性: Soluble in DMSO
- 保存条件: -20°C
- 配置溶液浓度参考:
1mg 5mg 10mg 1 mM 1.934 ml 9.67 ml 19.341 ml 5 mM 0.387 ml 1.934 ml 3.868 ml 10 mM 0.193 ml 0.967 ml 1.934 ml 50 mM 0.039 ml 0.193 ml 0.387 ml
- 注意:部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。
输入产品批号:
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)