AT-56

    
99%

AT-56

源叶(MedMol)
S88801 一键复制产品信息
162640-98-4
C25H27N5
397.52
货号 规格 价格 上海 北京 武汉 南京 购买数量
S88801-5mg 99% ¥200.00 7 - - -
S88801-10mg 99% ¥320.00 6 - - -
S88801-25mg 99% ¥600.00 货期:2-3天 - - -
S88801-50mg 99% ¥1050.00 货期:2-3天 - - -
S88801-100mg 99% ¥1800.00 货期:2-3天 - - -
产品介绍 参考文献 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

AT-56 是一种口服活性的 lipocalin-type prostaglandin (PG) D synthase (L-PGDS) 的选择性抑制剂,对应的Ki值和IC50值分别为75 μM和95 μM

产品描述: AT-56 是一种口服活性的 lipocalin-type prostaglandin (PG) D synthase (L-PGDS) 的选择性抑制剂,对应的Ki值和IC50值分别为75 μM和95 μM
靶点: L-PGDS (Cell-free assay):75 μM(Ki);L-PGDS(Cell-free assay):95 μM;PGESynthase
体内研究: Orally administered AT-56 (<30 mg/kg body weight) decreases the PGD2 production to 40% in the brain of H-PGDS-deficient mice after a stab wound injury in a dose-dependent manner without affecting the production of PGE2 and PGF2α and also suppresses the accumulation of eosinophils and monocytes in the bronco-alveolar lavage fluid from the antigen-induced lung inflammation model of human L-PGDS-transgenic mice.
细胞实验: Cell lines: TE-671 cells, MEG-01S cells Concentrations: 0 - 100 μM Incubation Time: 10 min Method: TE-671 (1×106 cells/well) and MEG-01S (5×105 cells/well) are seeded into multiplates and cultured in Dulbecco's modified Eagle's medium supplemented with 10% heat-inactivated fetal bovine serum, 4 mM L-glutamine, 4.5 g/liter glucose, 100 units/ml penicillin, and 100 μg/ml streptomycin sulfate under a 5% CO2 atmosphere at 37 ℃. MEG-01S cells are caused to differentiate by treatment with 12-O-tetradecanoylphorbol-13-acetate to express H-PGDS and COX-1. After the cells have been cultured for 1 day, AT-56 is added to them at different doses ranging from 0 to 100 μM, and the cells are then incubated at 37 ℃ for 10 min. Thereafter, they are stimulated with a calcium ionophore, A23187 (5 μM), at 37 ℃ for 15 min. The culture media were harvested and centrifuged at 10,000 × g for 5 min to remove the cells, and the supernatant is removed and stored at 80 ℃ until the measurements of PGs can be made.
动物实验: Animal Models: H-PGDS KO mice (14–16-week-old males or females weighing 25–30 g, C57BL/6 strain) Dosages: 1 mg/kg, 3 mg/kg, 10 mg/kg, 30 mg/kg Administration: Oral gavage
参考文献: 1. Daisuke Irikura, et al. J Biol Chem. 2009 Mar 20;284(12):7623-30.
溶解性: Soluble  in  DMSO
保存条件: -20℃
配置溶液浓度参考:
1mg 5mg 10mg
1 mM 2.516 ml 12.578 ml 25.156 ml
5 mM 0.503 ml 2.516 ml 5.031 ml
10 mM 0.252 ml 1.258 ml 2.516 ml
50 mM 0.05 ml 0.252 ml 0.503 ml
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参考文献

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