• 产品描述： Benpyrine racemate ((Rac)-Benpyrine) 是一种有效的、高特异性的 TNF-α 口服活性抑制剂，可减轻TNF-α诱导的炎症，从而减少肝和肺损伤

• 靶点： TNF-α;TNF

• 体内研究：
  Benpyrine attenuates TNF-α-induced inflammation in endotoxemic murine model, thereby reducing liver and lung injury, and significantly reliefs the symptoms of collagen-induced arthritis and imiquimod-induced psoriasiform inflammation in mice

• 细胞实验： Cell lines: RAW264.7 macrophages Concentrations: 5, 10 or 20 μM Incubation Time: 12 h Method: RAW 264.7 cells were grown to 50−70% confluence in a glass chamber, pretreated with DMSO or benpyrine for 12 h, and then stimulated with 10 ng/mL TNF-α for 2 h. Cell treatments were terminated by washing the cells with PBS, followed by fixation with freshly prepared 4% paraformaldehyde in PBS for 20 min. The fixed cells were washed three times with PBS and then permeabilized with 0.25% Triton X-100 in PBS for 10 min. After blocking with 2 mg/mL BSA for 1 h at room temperature, we added the NF-κB/p65 antibody at a 1:1000 dilution at 4 °C overnight, washed three times with PBS, then incubated with a 1:500 dilution of the secondary antibody for 1 h at room temperature in the dark. Finally, 4′,6-diamidino-2-phenyl indole (DAPI) was used to stain the nuclei at 37 °C for 30 min in the dark.

• 动物实验： Animal Models: CIA mice, Imiquimod-Induced Psoriasis-like Skin Lesions mice mode Dosages: 25, 50 mg/kg Administration: i.g.

• 参考文献：
  1. Weiguang Sun, et al. J Med Chem. 2020 Aug 13;63(15):8146-8156.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	3.243 ml	16.216 ml	32.432 ml
  5 mM	0.649 ml	3.243 ml	6.486 ml
  10 mM	0.324 ml	1.622 ml	3.243 ml
  50 mM	0.065 ml	0.324 ml	0.649 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

输入产品批号：

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *