• 产品描述： BCH001 是一种特殊的 PAPD5 抑制剂，可在先天性角化不良(DC)患者诱导的多能干细胞中恢复端粒酶活性和端粒长度。PAPD5 是一种具有非常规RNA结合基序的非规范化poly(A)聚合酶

• 靶点： PAPD5;DNA/RNASynthesis

• 体外研究：
  BCH001 is a specific PAPD5 inhibitor that restores telomerase activity and telomere length in DC patient induced pluripotent stem cells

• 细胞实验： Cell lines: CD34+ HSPCs, iPSCs and fibroblasts Concentrations: 1 μM, 5 μM, 100 μM Incubation Time: 72 h, 5 days, 14 days Method: Equal numbers of DC patient iPSCs are plated and 24 hr later treated with 1 μM BCH001 versus DMSO as vehicle control. 72 hr post-treatment cells are harvested and fixed using 70% ethanol at 4 ℃ overnight. For CD34+ HSPCs, 1000 cells are plated in methylcellulose medium containing human cytokines and inhibitors BCH001, RG7834 or vehicle (DMSO). After 14 days of culture at 37℃, 5% CO2, the progenitor colonies are counted manually under the microscope, with categories defined by colony morphology.

• 参考文献：
  1. Nagpal N, Wang J, Zeng J, Lo E, Moon DH, Luk K, Braun RO, Burroughs LM, Keel SB, Reilly C, Lindsley RC, Wolfe SA, Tai AK, Cahan P, Bauer DE, Fong YW, Agarwal S. Small-Molecule PAPD5 Inhibitors Restore Telomerase Activity in Patient Stem Cells. Cell Stem Cell. 2020 Jun 4;26(6):896-909.e8. doi: 10.1016/j.stem.2020.03.016. Epub 2020 Apr 21. PMID: 32320679; PMCID: PMC7275922.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.379 ml	11.895 ml	23.79 ml
  5 mM	0.476 ml	2.379 ml	4.758 ml
  10 mM	0.238 ml	1.19 ml	2.379 ml
  50 mM	0.048 ml	0.238 ml	0.476 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *