• 提示：详情请下载说明书。

• 产品描述： NIBR189 是 Epstein-Barr virus-induced gene 2 (EBI2, GPR183) receptor 的有效拮抗剂，其结合的IC50值为16 nM，其功能的IC50值为11 nM

• 靶点： EBI2 (function)(Cell-free assay):11 nM; EBI2 (binding)(Cell-free assay):16 nM;EBI2/GPR183

• 体外研究：
  NIBR189, the EBI2 antagonist, blocks the migration of U937 cells expressing functional EBI2 levels with an IC50 of 0.3 nM

• 体内研究：
  NIBR189 is a potent and selective EBI2 antagonist with pharmacokinetic properties which should allow use for in vitro and in vivo experiments

• 细胞实验： Cell lines: U937 cells Concentrations: 20 nM Incubation Time: 3 h Method: Prior to the migration assay, U937 cells were kept overnight in lipid-depleted media. Chemotaxis was performed using HTS Transwell-96 plates with 5.0 μm pore polycarbonate membranes. In brief, lower chambers were filled with 240 μL of compound solution in media in which the FCS was substituted with 1% BSA, and after insertion of the filters, 0.75×105 cells in 75 μL media were added to the upper chamber. After 3 h at 37℃, cells in the lower chamber were analyzed by flow cytometry.

• 动物实验： Animal Models: C57BL/6 male mice Dosages: 1 mg/kg (i.v.); 3 mg/kg (p.o.) Administration: i.v., p.o.

• 参考文献：
  1. Francois Gessier, et al. Identification and characterization of small molecule modulators of the Epstein-Barr virus-induced gene 2 (EBI2) receptor. J Med Chem. 2014 Apr 24;57(8):3358-68.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.329 ml	11.647 ml	23.293 ml
  5 mM	0.466 ml	2.329 ml	4.659 ml
  10 mM	0.233 ml	1.165 ml	2.329 ml
  50 mM	0.047 ml	0.233 ml	0.466 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

输入产品批号：

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *