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• 产品描述： RI-1 is a RAD51 inhibitor, with IC50s ranging from 5 to 30 μM. RI-1 binds covalently to the surface of RAD51 protein at cysteine 319. RI-1 inactivates RAD51 by directly binding to a protein surface that serves as an interface between protein subunits in RAD51 filaments. RI-1 can disrupt homologous recombination in human cells

• 靶点： IC50: 5-30 μM (RAD51);DNA/RNASynthesis

• 体外研究：
  RI-1 (1-50 µM; 24 h) specifically inhibits homologous recombination (HR) in U2OS cells and stimulates single-strand annealing (SSA) in HEK293 cells. RI-1 (5-20 µM; 30 min) inhibits HsRAD51 in a concentration-dependent manner. RI-1 (20 µM; 8 h) disrupts the formation of RAD51 foci after DNA damage in immortalized human fibroblasts. RI-1 (15-25 µM; 24 h) sensitizes human cancer cells (HeLa, MCF-7 and U2OS) to cross-linking chemotherapy

• 体内研究：
  RI-1 (50 mg/kg; i.p. every 3 d for 30 d) significantly reduces triple negative breast cancer (TNBC) tumor growth in mice. Animal Model: Female BALB/c nude mice (6 weeks) bearing TNBC tumor Dosage: 50 mg/kg Administration: I.p. every 3 days for 30 days Result: Resulted in significant inhibition of tumor growth.Did not cause body weight loss significantly.

• 参考文献：
  1. Budke B, et, al. RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells. Nucleic Acids Res. 2012 Aug;40(15):7347-57. 2. Shi Y, et, al. DAXX, as a Tumor Suppressor, Impacts DNA Damage Repair and Sensitizes BRCA-Proficient TNBC Cells to PARP Inhibitors. Neoplasia. 2019 Jun;21(6):533-544.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.765 ml	13.827 ml	27.654 ml
  5 mM	0.553 ml	2.765 ml	5.531 ml
  10 mM	0.277 ml	1.383 ml	2.765 ml
  50 mM	0.055 ml	0.277 ml	0.553 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *