• 提示：详情请下载说明书。

• 产品描述： Brequinar (DUP785, NSC 368390)是二氢乳清酸脱氢酶DHODH抑制剂，在体外IC50约为20 nM

• 靶点： DHODH:20 nM;VirusProtease; Dehydrogenase; DNA/RNASynthesis

• 体内研究：
  Brequinar sodium (BQR) induces anemia through inhibition of DHO-DHase activity and de novo pyrimidine synthesis in vivo. Coadministration of BQR with uridine prevents anemia

• 细胞实验： Cell lines: splenic T cells isolated from BALB/c mice Concentrations: 0.26, 1.3, 2.6 μM Incubation Time: 40-66 h Method: Lymphocytes are isolated from BALB/c spleen by centrifugation through a Ficol gradient and are depleted of macrophages and B cells by adherence to goat anti-mouse IgG-coated plates. The nonadherent cells are harvested and cultured at 4 to 5× 105/ml in RPMI 1640 supplemented with 10% FBS. Stimulation is performed with 5 μg/ml Con A in the presence of indicated concentrations of Brequinar or uridine. After 40 to 66 h, the cells are harvested and the levels of cell proliferation or purine NTP and PyNTP are quantified.

• 参考文献：
  1. Sykes DB, et al. Cell. 2016, 167(1):171-186.e15. 2. Xu X, et al. J Immunol. 1998 Jan 15;160(2):846-53.

• 溶解性： Soluble  in  DMSO

• 保存条件： 2-8℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.664 ml	13.32 ml	26.64 ml
  5 mM	0.533 ml	2.664 ml	5.328 ml
  10 mM	0.266 ml	1.332 ml	2.664 ml
  50 mM	0.053 ml	0.266 ml	0.533 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

输入产品批号：

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *