| 产品描述： | Micafungin Sodium is the sodium salt form of micafungin, a semi-synthetic echinocandin derived from a natural product of the fungus Coleophoma empetri with antifungal activity. |
| 靶点： |
Antibiotic;Antifungal |
| 体外研究： |
Micafungin (10 mg/mL) phenotypicly decreases the formation of biofilm in most of the isolates. For all the genes tested, the levels of mRNA transcription are also decreased significantly in micafungin-treated samples cf. their untreated counterparts. The combination of micafungin and KB425796-C is fungicidal and markedly reduces the number of CFU, in contrast to the fungistatic effects (no reduction in CFU) observed at all examined time points when each drug is used alone. |
| 体内研究： |
Micafungin (1 mg/kg) significantly prolongs survival compared with mice administered saline. Animals given a combination of micafungin (0.1 mg/kg) and KB425796-C (32 mg/kg) show a trend towards prolonged survival in comparison with those treated with micafungin (0.1 mg/kg) alone. In the livers of micafungin-treated mice, the number of CFUs decreases, although the clearance effect is less than that found in the kidneys. Combination treatment with micafungin and KB425796-C results in a significant decrease in the number of CFUs compared with the treatment with micafungin alone at all examined doses. The clearance effect associated with KB425796-C in combination with micafungin is greater than that observed in AMPH-treated animals. |
| 细胞实验： |
Each fungal isolate is incubated statically in yeast-maltose (YM) agar broth for 24 h at 30°C. Cryptococcus neoformans YC203 is grown in YM broth medium for 20 h at 30°C with shaking at 200 r.p.m. A cell suspension is prepared by washing the cultured cells once with sterile saline. A. fumigatus FP1305 is cultured on a potato dextrose agar (PDA) slant for 4 days, and spores are then harvested in sterile saline and collected by filtering through gauze. Antifungal activity against all isolates, with the exception of C. neoformans, is measured by the micro-broth dilution method in 96-well culture plates using RPMI 1640 medium supplemented with l-glutamine, but without sodium bicarbonate, and buffered to pH 7.0 with 0.165 m MOPS. ForC. neoformans, yeast nitrogen base-glucose (YNBD) medium is used. For the assay, the test microorganism is inoculated into each well to yield 1×105 CFU/well, and the plates are then incubated for 20 h or 48 h at 37°C. Two end points are determined by microscopic observation: MEC, which |
| 参考文献： |
1. Tsang C C, Tang J Y M, Ye H, et al. Rare/cryptic Aspergillus species infections and importance of antifungal susceptibility testing[J]. Mycoses. 2020, 63(12): 1283-1298. 2. Kai H, et al. Synergistic antifungal activity of KB425796-C in combination with micafungin against Aspergillus fumigat us and its efficacy in murine infection models. J Antibiot (Tokyo). 2013 Jun 12. 3. Bazzi W, et al. The inhibitory effect of micafungin on biofilm formation by Pseudomonas aeruginosa. Biofouling. 2013 Jul 23. 4. Tsang C C, Tang J Y M, Chan K F, et al. Diversity of phenotypically non-dermatophyte, non-Aspergillus filamentous fungi causing nail infections: importance of accurate identification and antifungal susceptibility testing[J]. Emerging microbes & infections. 2019;8(1):531-541. |
| 溶解性： |
Soluble in DMSO、H2O |
| 保存条件： |
2-8℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
0.774 ml |
3.869 ml |
7.738 ml |
| 5 mM |
0.155 ml |
0.774 ml |
1.548 ml |
| 10 mM |
0.077 ml |
0.387 ml |
0.774 ml |
| 50 mM |
0.015 ml |
0.077 ml |
0.155 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京