货号	规格	价格	上海	北京	武汉	南京
S80550-1mg	98%	￥230.00 S80550-1mg ￥230.00 现货	10 10	-	-	-
S80550-5mg	98%	￥700.00 S80550-5mg ￥700.00 现货	11 >10	-	-	-
S80550-25mg	98%	￥2500.00 S80550-25mg ￥2500.00 现货	6 6	-	-	-

产品介绍

Zotarolimus, an analogue of rapamycin, inhibits FKBP-12 (IC50= 2.8 nM).

产品描述：

Zotarolimus, an analogue of rapamycin, inhibits FKBP-12 (IC50= 2.8 nM).

靶点：

mTOR

体内研究：

Zotarolimus potently inhibits adjuvant DTH(ED50=1.72 mg/kg/day), EAE(ED50=1.17 mg/kg/day), and cardiac allograft rejection(ED50=3.71 mg/kg/day)

细胞实验：

Cell proliferation is assayed by measuring tritiated thymidine incorporation in vitro. Human coronary artery cells (hCa) are seeded into tissue culture flasks for expansion and applied to 96-well plates at desired density in complete media (5000 hCaSMC; 10 000 hCaEC). After 2 days, complete media is replaced with incomplete media to synchronize cells and induce G0 state. Two days later, incomplete media are removed and replaced with complete media (serum/growth factors) to induce G0 to G1 transition. Complete media also contain drug at desired concentrations to determine its effects on cell proliferation. On day 7, 3H-thymidine is added to cells to monitor DNA synthesis, and cells are harvested after overnight incorporation of radioactivity. After an incubation period of 72 h, 25 μL (1 μCi/well) of 3H-thymidine are added to each well. The cells are incubated at 37°C for 16-18 h to allow for incorporation of 3H-thymidine into newly synthesized DNA and the cells harvested onto 96-well plates containing bonded g

动物实验：

Male Sprague-Dawley rats was administrate by intravenous or oral Zotarolimus（2.5 mg/kg）dissolved inethanol: propylene glycol: cremophor EL: D5W vehicle (20: 30: 2: 48, by volume).

参考文献：

1.Chen YW, et al. J Cardiovasc Pharmacol, 2007, 49(4), 228-235. 2.Garcia-Touchard A, et al. Eur Heart J, 2006, 27(8), 988-1993.

溶解性：

Ethanol:93 mg/mL (96.3 mM) H2O:<1 mg/mL DMSO:93 mg/mL (96.3 mM)

保存条件：

2-8℃

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	1.035 ml	5.175 ml	10.35 ml
5 mM	0.207 ml	1.035 ml	2.07 ml
10 mM	0.103 ml	0.517 ml	1.035 ml
50 mM	0.021 ml	0.103 ml	0.207 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

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Zotarolimus