| 产品描述： | Azithromycin A is an antibiotic derived from erythromycin, binding to the 50S subunit of the bacterial ribosome, and thus inhibits translation of mRNA. |
| 靶点： |
Antibacterial;Antibiotic |
| 细胞实验： |
THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm. |
| 参考文献： |
1.Noedl H, et al. Antimicrob Agents Chemother. 2007 Feb;51(2):651-6. |
| 溶解性： |
DMSO:10 mM |
| 保存条件： |
2-8℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
1.361 ml |
6.803 ml |
13.606 ml |
| 5 mM |
0.272 ml |
1.361 ml |
2.721 ml |
| 10 mM |
0.136 ml |
0.68 ml |
1.361 ml |
| 50 mM |
0.027 ml |
0.136 ml |
0.272 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京