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• 产品描述： ICG-001 antagonizes Wnt/β-catenin/TCF-mediated transcription and specifically binds to element-binding protein (CREB)-binding protein (CBP) with IC50 of 3 μM, but is not the related transcriptional coactivator p300.

• 靶点： Wnt/beta-catenin;Epigenetic Reader Domain;EpigeneticReaderDomain;Wnt/beta-catenin

• 体内研究：
  PRI-724 exhibits antitumor activity in the mouse xenograft models of colon cancer. The initial results of the Phase I clinic trial of PRI-724 has been disclosed publically. The drug exhibits an acceptable toxicity profile with only one dose-limiting toxicity of grade 3 reversible hyperbilirubinaemia. An Open-Label dose-escalation phase I/II study of PRI-724 for patients with advanced myeloid malignancies is still ongoing

• 细胞实验： ICG-001 is dissolved in DMSO. To evaluate effects of ICG-001 on α-SMA and collagen type 1 expression, RLE-6TN cells are treated with TGF-β1 (0.25 ng/mL) in the presence or absence of ICG-001 (5.0 μM). After 24 h, cells are harvested and mRNA isolated for analysis by qPCR. RNA is reverse-transcribed using SuperScript reverse transcriptase. Quantitative PCR is performed with SYBR-Green PCR using Real-Time PCR System HT7900. The amplification protocol is set as follows: 95°C denaturation for 10 min followed by 40 cycles of 15-s denaturation at 95°C, 1 min of annealing/extension, and data collection at 60°C.

• 参考文献：
  1.Yosuke Osawa, et al. EBioMedicine. 2015, 2(11):1751-1758. 2.Liu F, Yu F, Lu Y Z, et al. Crosstalk between pleural mesothelial cell and lung fibroblast contributes to pulmonary fibrosis[J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. 2020, 1867(11): 118806. 3.Sasaki T, et al. Transl Respir Med. 2:8. 4.Zhang X, et al. Am J Cancer Res. 2015, 5(8):2344-2360.

• 溶解性： DMSO:54.9  mg/mL  (100  mM)    Ethanol:27.4  mg/mL  (50  mM)

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	1.823 ml	9.114 ml	18.227 ml
  5 mM	0.365 ml	1.823 ml	3.645 ml
  10 mM	0.182 ml	0.911 ml	1.823 ml
  50 mM	0.036 ml	0.182 ml	0.365 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *