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产品描述： Poziotinib(NOV120101; HM781-36B) is an irreversible HER1/2/4 inhibitor (IC50s: 3/5/23 nM). |
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靶点：
Apoptosis;EGFR;HER;Apoptosis; EGFR; HER |
体内研究：
Poziotinib对EGFR磷酸化和下游信号级联放大的关键组分有抑制作用,如STAT3,AKT和ERK,且对HER2扩增的胃癌细胞生长有特异性抑制效果。经激活HER2扩增的胃癌细胞中线粒体途径,Poziotinib还可诱导G1细胞周期阻滞和细胞凋亡。此外,在HER2诱发的和HER2非扩增的胃癌细胞中,Poziotinib与化疗剂有协同效果 |
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细胞实验：
Viable cell growth is determined by an MTT reduction assay. Briefly, all cell lines are seeded at a density of 3 × 103 per well in 96-well culture plates and then incubated at 37 °C for 24 h. The cells are then treated with 0.001, 0.01, 0.1, or 10 μM of HM781-36B. Three days later, 50 μg of MTT are added to each well and the samples are then incubated for 4 h to reduce the dye. Next, the samples are treated with DMSO, after which the absorbance of the converted dye in the living cells is measured at a wavelength of 540 nm. Six replicate wells are used for each analysis, and at least three independent experiments are conducted. Data points shown represent the mean while bars represent the SE.(Only for Reference) |
参考文献：
1.Nam HJ, et al. Cancer Lett. 2011, 302(2), 155-165. 2.Cha MY, et al. Int J Cancer. 2012, 130(10), 2445-2454. |
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溶解性：
H2O:<1 mg/mL DMSO:91 mg/mL (185.2 mM) Ethanol:<1 mg/mL |
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保存条件：
-20℃ |
配置溶液浓度参考：
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1mg |
5mg |
10mg |
| 1 mM |
2.035 ml |
10.176 ml |
20.352 ml |
| 5 mM |
0.407 ml |
2.035 ml |
4.07 ml |
| 10 mM |
0.204 ml |
1.018 ml |
2.035 ml |
| 50 mM |
0.041 ml |
0.204 ml |
0.407 ml |
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| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
北京