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• 产品描述： CAY10650 is a highly potent cytosolic phospholipase A2α (cPLA2α) inhibitor with an IC50 value of 12 nM. CAY10650 suppresses lipid droplets formation and PGE2 secretion

• 靶点： Phospholipase

• 体外研究：
  CAY10650 (12 nM; 30 min; neutrophils) inhibits the expression of the phosphorylated cPLA2-α (p-cPLA2-α) in cells.CAY10650 (12 nM; 2 h; neutrophils) inhibits PGE2 release in neutrophils. Western Blot Analysis Cell Line: Neutrophils Concentration: 12 nM Incubation Time: 30 minutes Result: Inhibited cPLA2-α and inhibited the expression of the p-cPLA2-α. Western Blot Analysis Cell Line: Neutrophils Concentration: 12 nM Incubation Time: 2 hours Result: Inhibited the PGE2 secretion and inhibited PGE2 realease.

• 体内研究：
  CAY10650 (50 μg/5 μL; Inject with topical eye-drop; Chinese hamsters) relieves acanthamoeba keratitis in vivo.. Animal Model: Chinese hamsters with infected with parasite-laden contact lenses Dosage: 50 μg/5 μL Administration: Inject with topical eye-drop under the contact lens; three times a day for 6 days and topically on days 7 to 20 postinfection Result: Reduced the severity of the keratitis and hasten the onset of resolution.Had little mild inflammation and very few PMNs infiltration in the corneal stroma.

• 参考文献：
  1. Paloschi MV, et, al. Cytosolic phospholipase A2-α participates in lipid body formation and PGE2 release in human neutrophils stimulated with an L-amino acid oxidase from Calloselasma rhodostoma venom. Sci Rep. 2020 Jul 3;10(1):10976. 2. Tripathi T, et, al. Role of phospholipase A₂ (PLA₂) inhibitors in attenuating apoptosis of the corneal epithelial cells and mitigation of Acanthamoeba keratitis. Exp Eye Res. 2013 Aug;113:182-91.

• 溶解性： soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.121 ml	10.604 ml	21.209 ml
  5 mM	0.424 ml	2.121 ml	4.242 ml
  10 mM	0.212 ml	1.06 ml	2.121 ml
  50 mM	0.042 ml	0.212 ml	0.424 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *