| 产品描述： | GC376 是一种 3CLpro (3C-like protease) 的抑制剂，对于PEDV 3CLpro的IC50值约为1.11 μM。GC376 对多种冠状病毒（包括SARS-CoV）的3CLpro有活性 |
| 靶点： |
PEDV 3CLpro(Cell-free assay):1.11 μM;SARS-CoV |
| 体外研究： |
Molnupiravir and GC376 show a synergistic activity on SARS-CoV-2 at 48 h, and an additive activity on SARS-CoV-2 at 72 h |
| 体内研究： |
GC376, a dipeptidyl bisulfite adduct salt, is a 3CLpro (3C-like protease) inhibitor that exerts strong inhibitory effects on picornaviruses and coronaviruses, including SARS-CoV. |
| 细胞实验： |
Cell lines: Vero E6 cells Concentrations: 0.21-16.7 μM Incubation Time: 48 and 72 h Method: Vero E6 cells (3000 cells/well) were seeded in 96-well clear flat-bottom plates and incubated at 37°C with 5% CO2 for 24 h. After incubation, cells were infected using a multiplicity of infection (MOI) of 0.1. SARS-CoV-2 was allowed to adsorb for one hour at 37°C. Subsequently, virus inoculum was removed, and cells were overlaid with media containing 3-fold serial dilutions of molnupiravir (0.62-50 μM), nirmatrelvir (0.62-50 μM) and GC376 (0.21-16.7 μM). Negative controls (compounds alone), infected positive controls (SARS-CoV-2 alone) and mock-infected cells were included in each plate. Plates were incubated at 37°C with 5% CO2 for 48 and 72 h and then, cell viability was measured using an MTT reduction assay. |
| 动物实验： |
Animal Models: Female BALB/c mice Dosages: 111 or 55.5 mg/kg Administration: i.m. |
| 参考文献： |
1. Gang Ye, et al. Structural Basis for Inhibiting Porcine Epidemic Diarrhea Virus Replication with the 3C-Like Protease Inhibitor GC376. Viruses. 2020 Feb 21;12(2):240. 2. Gidari A, et al. The Combination of Molnupiravir with Nirmatrelvir or GC376 Has a Synergic Role in the Inhibition of SARS-CoV-2 Replication In Vitro. Microorganisms. 2022 Jul 21;10(7):1475. 3. Shi Y, et al. The preclinical inhibitor GS441524 in combination with GC376 efficaciously inhibited the proliferation of SARS-CoV-2 in the mouse respiratory tract. Emerg Microbes Infect. 2021 Dec;10(1):481 |
| 溶解性： |
Soluble in DMSO、Ethanol |
| 保存条件： |
-20℃，充氮保存 |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
1.97 ml |
9.852 ml |
19.703 ml |
| 5 mM |
0.394 ml |
1.97 ml |
3.941 ml |
| 10 mM |
0.197 ml |
0.985 ml |
1.97 ml |
| 50 mM |
0.039 ml |
0.197 ml |
0.394 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京