| 产品描述： | Y16 is a specific inhibitor of Leukemia-associated Rho guanine nucleotide exchange factor (LARG) with a Kd value of 76 nM. Y16 is active in blocking the interaction of LARG and related G-protein-coupled Rho GEFs with RhoA. Y16 shows no detectable effect on other diffuse B-cell lymphoma (Dbl) family Rho GEFs, Rho effectors, or a RhoGAP |
| 靶点： |
Kd: 76 nM (LARG);Rho;Ras |
| 体外研究： |
Y16 (10-30 μΜ; 24 hours; NIH 3T3 cells) could inhibit RhoA-GTP formation induced by serum dose dependently and is specific for RhoA. Y16 (10-30 μΜ; 24 hours; NIH 3T3 cells) efficiently inhibits serum or SDF-1α-induced phospho-MLC and phospho-FAK formation, which are downstream of RhoA. Cell Viability Assay Cell Line: NIH 3T3 cells Concentration: 10 μΜ, 30 μΜ Incubation Time: 24 hours Result: Inhibited RhoA-GTP formation induced by serum dose dependently and was specific for RhoA. Western Blot Analysis Cell Line: NIH 3T3 cells Concentration: 10 μΜ, 30 μΜ Incubation Time: 24 hours Result: Inhibited serum or SDF-1α-induced phospho-MLC and phospho-FAK formation, which were downstream of RhoA. |
| 参考文献： |
1. Shang X, et al. Small-molecule inhibitors targeting G-protein-coupled Rho guanine nucleotide exchange factors. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3155-60. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-20℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.601 ml |
13.006 ml |
26.013 ml |
| 5 mM |
0.52 ml |
2.601 ml |
5.203 ml |
| 10 mM |
0.26 ml |
1.301 ml |
2.601 ml |
| 50 mM |
0.052 ml |
0.26 ml |
0.52 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京