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产品描述： UF010是I类HDAC选择性抑制剂，对HDAC1、HDAC2、HDAC3、HDAC8、HDAC6和HDAC10的IC50值分别为0.5 nM、0.1 nM、0.06 nM、1.5 nM、9.1 nM和15.3 nM |
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靶点：
HDAC3(Cell-free assay):0.06 nM; HDAC2(Cell-free assay):0.1 nM; HDAC1(Cell-free assay):0.5 nM; HDAC8(Cell-free assay):1.5 nM; HDAC6(Cell-free assay):9.1 nM; HDAC10:15.3 nM;HDAC |
体外研究：
In Cell-cycle analysis using MDA-MB-231 cells, UF010 mainly blocks G1/S transition with an increased G1 cell population and a reduced cell population in the S phase in a dose-dependent manner. UF010 has a half-life of 15.8 hr in cell culture medium containing 10% fetal bovine serum |
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细胞实验：
Cell lines: HCT116 cells and A549 cells Concentrations: 2 μM Incubation Time: 1 h Method: HCT116 and A549 cells are exposed to DMSO or etoposide (Etop, 10 μM) for 6 hr. TSA (0.2 μM), MS-275, and UF010 (2 μM) are added 1 hr before cell lysis. The total cell lysates are subject to western blotting with antibodies to the indicated proteins. PCNA is detected as a loading control. |
参考文献：
1. Wang Y, et al. Identification of histone deacetylase inhibitors with benzoylhydrazide scaffold that selectively inhibit class I histone deacetylases. Chem Biol. 2015, 22(2):273-84. |
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溶解性：
Soluble in DMSO、Ethanol |
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保存条件：
-20℃ |
配置溶液浓度参考：
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1mg |
5mg |
10mg |
| 1 mM |
3.688 ml |
18.44 ml |
36.879 ml |
| 5 mM |
0.738 ml |
3.688 ml |
7.376 ml |
| 10 mM |
0.369 ml |
1.844 ml |
3.688 ml |
| 50 mM |
0.074 ml |
0.369 ml |
0.738 ml |
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| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
北京