• 产品描述： Lobaplatin (D-19466) is a diastereometric mixture of platinum(II) complexe. Lobaplatin arrests cell cycle at G1 and G2/M phase. Lobaplatin induces apoptosis by increasing expressions of caspase and Bax, decreasing expression of Bcl-2. Lobaplatin can be used for research of cancer

• 靶点： ApoptosisCaspase;Bcl-2 Family;DNAAlkylation

• 体外研究：
  Lobaplatin (D-19466, 0.25-32 μg/mL; 24-72 h) exhibits anti-proliferative activities against esophageal squamous cell carcinoma (ESCC) cell lines. Lobaplatin (0-16 μg/mL; 48 h) induces esophageal squamous cell carcinoma (ESCC) apoptosis and modulates expression of apoptosis-related proteins. Lobaplatin (1.45 μg/mL; 0-48 h; SMMC-7721 cells) arrests cell cycle progression at G1 and G2/M phases in a time-dependent manner. Lobaplatin (1.45 μg/mL; 0-48 h; SMMC-7721 cells) inhibits the mRNA levels of cyclin B, CDK1, and CDC25C phosphatase, down-regulates Rb/E2F complexes and up-regulates of CDK inhibitors. Cell Viability Assay Cell Line: KYSE-410 cells and EC-109 cells Concentration: 0.25, 0.5, 1, 2, 4, 8, 16 and 32 μg/mL Incubation Time: 24, 48 and 72 hours Result: Inhibited the growth of KYSE-410 and EC-109 cells in a dose- and time-dependent manner.Inhibited the clone formation activity of KYSE-410 and EC-109 cells in a dosedependent manner. Apoptosis Analysis Cell Line: KYSE-410 cells and EC-109 cells Concentration: 0.25, 1, 4 and 16 μg/mL Incubation Time: 48 hours Result: Increased the percentage of apoptotic cells in a dose-dependent manner. Western Blot Analysis Cell Line: KYSE-410 cells and EC-109 cells Concentration: 0, 1, 4 and 16 μg/mL Incubation Time: 48 hours Result: Increased expressions of cleaved-caspase-3, cleaved-caspase-8, cleaved-caspase-9 and Bax, while decreased expression of Bcl-2. Cell Cycle Analysis Cell Line: SMMC-7721 cells Concentration: 1.45 μg/mL Incubati

• 体内研究：
  Lobaplatin (5 and 10 mg/kg; i.p.; once a week, for 3 weeks) suppresses tumor growth of esophageal squamous cell carcinoma (ESCC) xenograft. Animal Model: Male BALB/c nude mice (4-6 weeks) with ESCC xenograft Dosage: Intraperitoneal injection; once a week, for 3 weeks Administration: 5 and 10 mg/kg Result: Suppressed tumor volumes in a dose-dependent manner.Increased expressions of Bax and decreased expressions of Bcl-2.

• 参考文献：
  1. Du L, et, al. Antitumor activity of Lobaplatin against esophageal squamous cell carcinoma through caspase-dependent apoptosis and increasing the Bax/Bcl-2 ratio. Biomed Pharmacother. 2017 Nov;95:447-452. 2. Wu Q, et, al. Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells. J Hematol Oncol. 2010 Oct 31;3:43. 3. McKeage MJ. Lobaplatin: a new antitumour platinum drug. Expert Opin Investig Drugs. 2001 Jan;10(1):119-28.

• 溶解性： Soluble  in  H2O

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.517 ml	12.584 ml	25.169 ml
  5 mM	0.503 ml	2.517 ml	5.034 ml
  10 mM	0.252 ml	1.258 ml	2.517 ml
  50 mM	0.05 ml	0.252 ml	0.503 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *